Fluorometric estimation of GSH-OPT

McNeil, Thomas L.; Beck, Lyle V.

doi:10.1016/0003-2697(68)90285-6

Cited by 49 publications

(18 citation statements)

References 3 publications

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“…The total protein concentration was determined using CBB (Coomassie brilliant blue G250) staining, and the results were expressed as micromole per gram wet tissue protein of lung (μmol/g protein). Reduced glutathione (GSH) in lung tissue was measured fluorometrically as described previously (24,25). Briefly, a 200-μl BALF sample was mixed with an equal amount of 10% TCA and then centrifuged at 5000 x g for 10 min.…”

Section: Materials and Animalsmentioning

confidence: 99%

Therapeutic effects of α-lipoic acid on bleomycin-induced pulmonary fibrosis in rats

Liu

Ahmed²,

Nantajit³

et al. 2007

Int J Mol Med

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Abstract. Pulmonary fibrosis (PF) is a major side effect of radiotherapy and chemotherapy. Recent clinical trials, unfortunately, have failed to identify any therapeutic agent which has the potential to reduce the consequences of this devastating condition. Reactive oxygen species and tissue remodeling regulators, such as metalloproteinases (MMPs) and their inhibitors (TIMPs), are thought to be involved in the development of PF. We investigated these factors to determine the protective effects of antioxidant α-lipoic acid (LA) against antineoplastic agent bleomycin (BLM)-induced oxidant lung toxicity in Sprague-Dawley rats. At different time intervals after BLM administration, pathological changes of the lung were analyzed with the measurement of total protein in bronchoalveolar lavage fluid (BALF), hydroxyproline (HYP) content and the level of three oxidative stress markers, i.e. malondialdehyde (MDA), the GSH/GSSG ratio, and total antioxidative capability (T-AOC). Also, the expression changes of MMP-1 and TIMP-1 were measured. At day 14 or 28 after BLM administration, protein content in BALF, and HYP, MDA and T-AOC contents of the lung increased significantly with a decreased GSH/GSSG ratio, implicating an increased efflux of GSSG from the lung and consumption of GSH. In contrast, treatment with LA protected BLM-induced pulmonary injury by suppressing oxidative stress with the reduction of MDA, and the enhancement of the GSH/GSSG ratio and T-AOC. The BLM-stimulated symptoms of PF were relieved with significant reduction of HYP and total proteins in LA-treated rats. LA also ameliorated the MMP-1/TIMP-1 ratio. These results suggest that LA inhibits BLM-induced lung toxicity associated with oxidative damage. Therefore, antioxidant LA has a potential therapeutic effect in the prevention and alleviation of PF.

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Section: Materials and Animalsmentioning

confidence: 99%

Therapeutic effects of α-lipoic acid on bleomycin-induced pulmonary fibrosis in rats

Liu

Ahmed²,

Nantajit³

et al. 2007

Int J Mol Med

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“…GSH in the extract was determined fluorometrically according to the method of McNeil and Beck (1968).…”

Section: Determination Of Gshmentioning

confidence: 99%

Possible involvement of active oxygen species in selenite toxicity in isolated rat hepatocytes

1993

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Mechanisms of selenite cytotoxicity were examined using isolated rat hepatocytes. When selenite was added to a suspension of rat hepatocytes, intracellular reduced glutathione (GSH) was decreased and the oxygen consumption rate was increased. Subsequently, thiobarbituric acid-reactive substances (TBA-RS) and lactate dehydrogenase (LDH) leakage were increased. A ferric iron chelator, desferrioxamine (DF), and a synthetic superoxide dismutase (SOD) mimic, desferrioxamine manganese (DFMn), reduced the selenite toxicity. These results suggest that superoxide anion and its reactive metabolites such as the hydroxyl radical may be involved in the cytotoxicity of selenite.

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“…The level of reduced and oxidized glutathione in plasma was determined as described elsewhere (Ellman and Lysko, 1967;McNell and Beck, 1968) with modifications. Glutathione level was determined in 100 µ l plasma at 350/420 nm.…”

Section: Methodsmentioning

confidence: 99%

Response of the glutathione system to chronic irradiation of human population after the Chernobyl accident

Ivanenko

Burlakova

2005

Biol Bull Russ Acad Sci

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A complex relationship between glutathione level in plasma in human population (children living in radionuclide-contaminated regions and the Chernobyl liquidators) exposed to chronic low-level radiation after the Chernobyl accident was demonstrated. The obtained experimental data indicate different responses of the human glutathione system to low (from 0.1 to 20 cSv) and high (from 20 to 150 cSv) doses of ionizing radiation.

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Fluorometric estimation of GSH-OPT

Cited by 49 publications

References 3 publications

Therapeutic effects of α-lipoic acid on bleomycin-induced pulmonary fibrosis in rats

Therapeutic effects of α-lipoic acid on bleomycin-induced pulmonary fibrosis in rats

Possible involvement of active oxygen species in selenite toxicity in isolated rat hepatocytes

Response of the glutathione system to chronic irradiation of human population after the Chernobyl accident

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