Fluorometric determination of selenium in sea-water

Flotation separation and atomic absorption spectrometric determination of selenium(IV) in water

“…According to recent reliable data (3,16,18), the concentrations of selenium(IV) in uncontaminated natural fresh and seawaters are quite low, <5-70 ng/L. Shimoishi and Tóei (18) who used gas chromatographic determination have reported that the coastal seawater and river water of Japan was found to contain 8-32 ng/L of selenium(IV) and that the selenium(IV) content in seawater collected at the seashore of Shibukawa, Okayama Prefecture, Japan, was 32 ng/L.…”

“…Iron(III) hydroxide is well known as one of the most efficient collectors of trace metals in aqueous systems. As a preconcentration technique for the determination of selenium(IV) in water, coprecipitation with iron(III) hydroxide is commonly used (1)(2)(3). This bulky amorphous precipitate, however, is difficult to filter, and centrifugation is cumbersome for larger volumes.…”

High-performance liquid chromatography-fluorometric determination of selenium based on selenotrisulfide formation reaction

“…Therefore, the biological and environmental investigations of selenium demand a sensitive and reliable method for the determination of low levels of selenium. Fluorometry (5,6), atomic absorption spectrometry (7,8), inductively coupled plasma emission spectrometry (9), neutron activation analysis (10), and gas chromatography (11) have so far been appreciated for this purpose. The fluorometric method based on the formation of piazselenol from selenium (IV) and 2,3-diaminonaphthalene (DAN) (5) has been widely used owing to its low detection limit.…”

“…Fluorometry (5,6), atomic absorption spectrometry (7,8), inductively coupled plasma emission spectrometry (9), neutron activation analysis (10), and gas chromatography (11) have so far been appreciated for this purpose. The fluorometric method based on the formation of piazselenol from selenium (IV) and 2,3-diaminonaphthalene (DAN) (5) has been widely used owing to its low detection limit. However it needs complicated procedures to cleanup the reagent and to extract the fluorophore, and further purification of the fluorophore was needed for precise determination of selenium in biological sample (6,12).…”