Fluorometric Determination of Ethinyl Estradiol in Tablets

Rapid, Sensitive Colorimetric Method for Determination of Ethinyl Estradiol

“…The sensitivity of the method is such that single-tablet analysis, even at the lowest dosage, is feasible; this sensitivity is an improvement over the diazotized 4-amino-6-chloro-m -benzenedisulfonamide method (14). Another advantage is the applicability of the method to progestin-estrogen combinations, which is an improvement over the fluorometric method (15).…”

“…In spite of its high sensitivity, the Liebermann-Burchard reaction-based fluorometric method of James (15), with 0.5 bg/ml as a lower limit for detection, is not applicable to progestin-estrogen preparations. Progestational steroids produce a color in the Liebermann-Burchard reaction that quenches the fluorescence of ethinyl estradiol.…”

Determination of Estrogens in Dosage Forms by Fluorescence Using Dansyl Chloride

“…At this low estrogen level, the behavior may be due in part to the interference of tablet excipients and excess dansyl chloride reagent. The method of James (39) is suggested as an alternative.…”

Sensitive Voltammetric Determination of Ethinyl Estradiol on Carbon Black Modified Electrode

“…5 EE is characterized by an extremely high physiological activity, and primarily because of this properties is an object of numerous studies considering its influence on people and environment. 6 Many analytical methods have been established for ethinyl estradiol determination, including spectrophotometry, [7][8][9] gas and liquid chromatography coupled with mass spectrometry, 6,[10][11][12][13][14][15] fluorometry, 16 immunoassay methods, 17 argentometry, 18 amperometry, 19 electrophoresis, 20 isotope dilution mass spectrometry 21 or micellar electrokinetic capillary chromatography. 22,23 However, many of these methods are usually complex and time consuming.…”