Fluorometric Determination of Estradiol Valerate in Sesame Oil or Ethyl Oleate Injectables

Abstract

A fluorometric procedure has been developed for the quantitative determination of estradiol valerate in sesame oil or ethyl oleate. An n-heptane solution of the steroid is purified, using a nitromethane partition column. The purified estradiol valerate is dissolved in alcohol and determined fluorometrically. The method is applicable to a variety of commercial preparations, including estradiol valerate-testosterone enanthate combinations. A total of 14 determinations on 5 different synthetic preparations gave an average recovery of 97.2%. It is recommended that this method be subjected to a collaborative study.

Electrochemical sensor highly selective for estradiol valerate determination based on a modified carbon paste with iron tetrapyridinoporphyrazine

“…6,7 A large body of work has reported the use of different analytical techniques for the quantification of these estrogenic hormones. [8][9][10][11][12][13] Electrochemical detection of these analytes using sensors [14][15][16] and biosensors 17 is generally limited to the natural hormone estradiol and, to our knowledge, no articles using chemically modified electrodes have been described in the literature for synthetic hormones like estradiol valerate (EV). The unique electroanalytical method reported for the sensitive EV determination is based on use of a static mercury drop electrode at a potential of À1.29 V vs. SCE.…”