Fluorometric Determination of Estradiol Valerate in Sesame Oil or Ethyl Oleate Injectables. II. Collaborative Study

Abstract

A fluorometric method for determining estradiol valerate in sesame oil or ethyl oleate was subjected to collaborative study. The method utilizes a nitromethane-Celite column to isolate and purify the steroid, which is then measured fluorometrically. Four commercial samples and 3 synthetic samples, varying in concentration from 4 to 20 mg/ml, were used in the study. The collaborative results were acceptable: Coefficients of variation ranged from 2.48 to 3.55%; average recoveries for the synthetics ranged from 98.6 to 102%. The method has been adopted as official first action.

Determination of estradiol valerate in pharmaceutical preparations and human serum by flow injection chemiluminescence

“…Interest in estradiol valerate has significantly increased in the past few decades. As a result, a large number of analytical methods have been developed for the quantification of EV such as spectrophotometry , electrochemical methods , fluorescence spectrometry , chromatography , gas chromatography mass spectrometry (GC‐MS) and electrochemical sensing .…”

Simultaneous Spectrophotometric Determination of Estradiol Valerate and Cyproterone Acetate : Estradiol Valerat Ve Siproteron Asetat'in Bi̇r Arada Spektrofotometri̇k Tayi̇ni̇

“…Various methods have been reported on the determination of estradiol derivatives and cyproterone acetate. These are colorimetry (3), fluorometry (4,5), GC (6), GC-MS (7), RIA (8), HPLC (9)(10)(11)(12). But no references were cited for the simultaneous determination of these drugs.…”