Fluorometric determination of demethylchlortetracycline and tetracycline in mammalian bone

Simple and rapid methods for the determination and detection of autofluorescence and oxytetracycline (OTC) induced fluorescence in powdered and intact bone (without previous chemical extraction of the antibiotic) were used to estimate age and sex related differences in the rat femur. The autofluorescence always had collagen characteristics; it increased with age and was not sex dependent. The intensity of OTC induced fluorescence decreased with age, being higher in males than in females. In the oldest animals, however a slight increase in the fluorescence intensity was observed, and sex differences disappeared. The spectrofluorometric measurements of intact femora indicate a higher OTC retention on the bone surface of young animals and adult males but not in adult females. The results indicate that the determination of OTC retention in the bone using our quantitative fluorometric method and the detection of OTC deposition at bone surface using a spectrofluorometric technique can be a sensitive and inexpensive tool for experimental studies of bone changes in physiological and pathological conditions.

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Schnelle fluorimetrische Bestimmung von Chlortetracyclin in Fleisch und Knochen

Honikel¹,

Hambloch²

1976

Z Lebensm Unters Forch

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Antibiotics in biological materials, including tetracyclines, are determined usually be means of microbiological methods. Because of the low specificity and the time consuming procedure of the microbiological assays, we developed a fluorimetric method, which enables us to determine specifically the tetracyclines and to reduce the time for the assay down to 4 hours. Our method is based on the determination of tetracyclines published by Kohn (1961), which we modified for the determination of chlortetracycline in meat and bone. Tetracyclines are fluorescent compounds, whose quantum yields are very much enhanced in most cases by forming a complex with Ca2+ ions and by extracting it into an organic solvent. This transfer into acetic acid ethylester separates the chlortetracycline from interfering substances. The yield of chlortetracycline with the described method was about 80%, the sensitivity of the detection was about 0.5 ppm.

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Fluorometric determination of demethylchlortetracycline and tetracycline in mammalian bone

Cited by 6 publications

References 4 publications

Mode of Action, Biotransformation and Pharmacokinetics of Antituberculosis Drugs in Animals and Man

Mode of Action, Biotransformation and Pharmacokinetics of Antituberculosis Drugs in Animals and Man

Determination of oxytetracycline induced fluorescence and autofluorescence as an indicator of age and sex differences in rat femur

Schnelle fluorimetrische Bestimmung von Chlortetracyclin in Fleisch und Knochen

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