Fluorometric Determination of Acetylsalicylic Acid and Salicylic Acid in Blood

Lange, Winthrop E.; Bell, Saul A.

doi:10.1002/jps.2600550407

Cited by 32 publications

(8 citation statements)

References 4 publications

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“…Also note that 0.1 mM aspirin is sufficient to block 0.5 U thrombin-induced platelet MDA production but it does not interfere with the inhibitory effect of the endothelium. Pharmacologically, this 0.1-mM aspirin concentration to the 0.12 mM plasma concentration of unchanged aspirin observed 15 min after the ingestion of 650 mg aspirin (two 5-grain tablets) (37) or 30 min after the ingestion of 1,300 mg aspirin (38). These plasma aspirin concentrations are 68% of that observed when the same dose is given intravenously (37).…”

Section: Resultsmentioning

confidence: 83%

Effect of Aspirin on Thrombin-Induced Adherence of Platelets to Cultured Cells from the Blood Vessel Wall

Czervionke¹,

Hoak²,

Fry³

1978

J. Clin. Invest.

191

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A B S T R A C T An in vitro method was used to detect adherence of 51Cr-labeled platelets to monolayers of cultured human endothelial, fibroblast, and smooth muscle cells. Washed platelets did not adhere to untreated or aspirin-treated endothelial monolayers in the absence of thrombin. In contrast, thrombin-induced platelet aggregates adhered to all of the monolayers but adherence to endothelium was significantly less than to the other cells. Additional evidence for adherence of platelets to the endothelium was provided by scanning and transmission electron microscopy. Thrombin-induced platelet adherence to endothelium was inhibited by hirudin. Platelet adherence induced by thrombin was enhanced significantly by treatment of the endothelial monolayer with 1-2 mM aspirin. This increase in adherence was seen even when aspirin-treated platelets were used; adherence values approached those seen with fibroblasts and smooth muscle cells. An aspirin concentration of 0.1 mM was sufficient to block thrombin-induced malonaldehyde production in platelets but it did not interfere with the inhibitory effect of the endothelium against platelet adherence. The effect of aspirin on the endothelium was temporary and inhibitory activity of the endothelium was restored 1 h after aspirin had been removed from the incubation system. The ability of thrombin to cause adherence of platelets to undamaged endothelium, and the potential for aspirin to enhance this adherence have implications for mechanisms which operate in platelet interaction with the blood vessel wall.

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Section: Resultsmentioning

confidence: 83%

Effect of Aspirin on Thrombin-Induced Adherence of Platelets to Cultured Cells from the Blood Vessel Wall

Czervionke¹,

Hoak²,

Fry³

1978

J. Clin. Invest.

191

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show abstract

“…Because aspirin rapidly hydrolyzes to salicylic acid in vivo (Lange and Bell, 1966;Cotty and Ederma, 1966;Morgan and Truitt, 1965), it may contribute to prevent Alzheimer's disease as salicylic acid. However, ibuprofen is easily metabolized to the hydroxy-and carboxy-derivatives by P450 (Sanins et al, 1990).…”

Section: Discussionmentioning

confidence: 99%

Inactivation of cholinesterase induced by non-steroidal anti-inflammatory drugs with horseradish peroxidase: Implication for Alzheimer's disease

Muraoka

Miura

2009

Life Sciences

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“…Several methods have been developed for the determination of this compound and its degradation by-product in vitro and in vivo. These methods include chromatography (Pereira et al 1998;Verstraeten, Roets, and Hoogmartens 1987;Bevitt, Mather, and Sharman 1984;Fogel, Epstein, and Chen 1984;Reepmer and Kichhoefer 1979;Ali 1976), fluorometry (Karim et al 2006;Miles and Schenk 1970;Lange and Bell 1966), colorimetry (Farid et al 1975;Trinder 1954), absorptiometry (Quinn 1994;Kitamura, Takagi, and Hozumi 1984;Kitamura and Majuna 1983;Fernandez and Klappmeier 1978), and voltammetry and potentiometry (Fernandez et al 1998;Fung and Luk 1989;Hassan and Hamada 1988). However, these methods require prior treatment and time-consuming steps (Pereira, Aniceto, and Fatibello-Filho 1998;Verma and Jain 1986;Juhl and Kichhhofer 1980;Routh et al 1967;Levine 1961;Brodie, Udenfriend, and Coburn 1944) and often involve an indirect determination through the determination of salicylic acid (SA).…”

Section: Introductionmentioning

confidence: 95%

Absorptiometric Determination of Acetylsalicylic Acid in Aqueous Ethanolic Solution

Iwunze

2008

Analytical Letters

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Fluorometric Determination of Acetylsalicylic Acid and Salicylic Acid in Blood

Cited by 32 publications

References 4 publications

Effect of Aspirin on Thrombin-Induced Adherence of Platelets to Cultured Cells from the Blood Vessel Wall

Effect of Aspirin on Thrombin-Induced Adherence of Platelets to Cultured Cells from the Blood Vessel Wall

Inactivation of cholinesterase induced by non-steroidal anti-inflammatory drugs with horseradish peroxidase: Implication for Alzheimer's disease

Absorptiometric Determination of Acetylsalicylic Acid in Aqueous Ethanolic Solution

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