Fluorometric Analysis of Selenium in Plants

Olson, O. E.

doi:10.1093/jaoac/52.3.627

Cited by 73 publications

(48 citation statements)

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“…The edible portions of two dozen nuts in each of the above categories were individually subdivided in a small food processor and subsampled for determination of selenium. The method of analysis involved wet digestion of the sample with nitric and perchloric acids and measurement of the fluorescence of piazselenol resulting from the reaction of selenium with 2,3-diaminonaphthalene (Olson 1969).…”

Section: Experimental Methodsmentioning

confidence: 99%

Variation in the Selenium Content of Individual Brazil Nuts

Secor

Lisk

1989

Journal of Food Safety

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Brazil nuts are high in selenium content relative to other human foods. Since the limits between human selenium essentiality and toxicity are narrow, it is important to know the variation in selenium content of individual nuts. Analysis was performed on 72 individual nuts obtained in stores as shelled nuts in bulk and shelled and unshelled nuts in packages. Their average selenium content was 14.66 ppm with a range of 0.2 to 253.0. The agronomic and health aspects of these findings are discussed.

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Section: Experimental Methodsmentioning

confidence: 99%

Variation in the Selenium Content of Individual Brazil Nuts

Secor

Lisk

1989

Journal of Food Safety

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“…Se was determined according to OLSON (15) and GSH-Px according to PAGLIA and VALENTINE (16) with cumene peroxide as reagent.…”

Section: Methodsmentioning

confidence: 99%

Selenium and glutathione peroxidase in blood and tissues and growth and feed efficiency in young bulls at different dietary selenium levels

Pehrson

Johnsson

1985

Zentralblatt für Veterinärmedizin Reihe A

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Summary Young bulls were given dietary levels of 0.08, 0.18, 0.29, 0.42, 0.58 and 1.08 mg Se per kg dry matter (ppm). There were no significant differences in growth and feed efficiency between the groups. Se content in whole blood and GSH‐Px activity in erythrocytes increased with dietary Se level. Se in the liver increased with Se level in feed and there was a tendency to increase also in GSH‐Px. In the kidney, Se and GSH‐Px were higher at 0.18 than at 0.08 ppm, but no further increase occurred at levels above 0.18 ppm. In myocardium there was an increase both of Se and GSH‐Px up to the dietary level of 0.58 ppm. In skeletal muscle the Se and GSH‐Px were significantly higher at 0.18 than at 0.08 ppm. From 0.18 ppm onwards the GSH‐Px levelled off, whereas Se tended to increase further, at least up to 0.42 ppm. In conclusion, it seems reasonable to give a figure of 0.10–0.15 ppm for the Se‐requirement of cattle. Zusammenfassung Selen und Glutathion‐Peroxidase in Blut und Geweben sowie Wachstum und Futterverwertung von Jungbullen bei Rationen mit unterschiedlichem Selengehalt Jungbullen erhielten Rationen mit einem Selengehalt von 0,08, 0,18, 0,29, 0,42, 0,58 und 1,08 mg Selen pro kg Trockensubstanz (ppm). Zwischen den Gruppen wurden keine signifikanten Unterschiede bezüglich Wachstumsrate und Futterverwertung beobachtet. Der Selengehalt im Vollblut und die GSH‐Px‐Aktivität in den Erythrozyten nahmen mit steigendem Selengehalt der Diät zu. In der Leber stieg der Selengehalt mit der Selenkonzentration im Futter an; ebenso bestand die Tendenz einer Zunahme der GSH‐Px. In den Nieren waren Se und GSH‐Px bei 0,18 ppm höher als bei 0,08 ppm, es kam jedoch bei höheren Konzentrationen zu keinem weiteren Ansteigen. Im Myocard wurde bis zu Rationen mit 0,58 ppm eine Zunahme des Selengehalts und der GSH‐Px beobachtet. In der Skelettmuskulatur lagen Se und GSH‐Px bei Rationen mit 0,18 ppm höher als bei 0,08 ppm. Von 0,18 ppm an kam es zu einer Plateaubildung bezüglich der GSH‐Px, während der Selengehalt mindestens bis zu 0,42 ppm weiter Anstieg. Es wird gefolgert, daß ein Selengehalt von 0,10 bis 0,15 ppm im Futter eine brauchbare Empfehlung hinsichtlich der Rationen für Rinder darstellt. Résumé Sélénium et glutathion‐peroxydase dans le sang et les tissus ainsi que la croissance et l'assimilation alimentaire chez des taurillons recevant des régimes à taux de sélénium différents Des taurillons ont reçu des rations avec un taux de sélénium de 0,08, 0,18, 0,29, 0,42, 0,58 et 1,08 mg de sélénium par kg de substance sèche (ppm). Aucune différence significative concernant le taux de croissance et l'assimilation alimentaire n'a été observée entre les groupes. Le taux du sélénium dans le sang et l'activité de GSH‐Px dans les érythrocytes ont augmenté avec le taux croissant de sélénium dans le régime. Le taux de sélénium dans le foie a augmenté avec la concentration du sélénium dans l'aliment; il y a eu également une tendance à l'augmentation de GSH‐Px. Se et GSH‐Px furent plus élevés dans les reins avec 0,18 ppm qu'avec 0,08 ppm, mai...

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“…The crops were air-dried at a temperature below 40°C to prevent loss of selenium, ground to a fine powder, mixed by tumbling and subsampled for elemental analysis. Following wet ashing with nitric and perchloric acids, selenium was determined by measuring the fluorescence of the complex formed between selenium and diaminonaphthalene (Olson 1969). For analysis of 19 other elements, including boron and molybdenum, the samples were first dry ashed at 450°C for six hours, digested with hydrogen peroxide and ashed at 450 "C for two hours.…”

Section: Methodsmentioning

confidence: 99%

Elemental Analysis of Grasses and Legumes Growing on Soil Covering Coal Fly Ash Landfill Sites

Weinstein

Osmeloski

Rutzke

et al. 1989

Journal of Food Safety

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Forage grasses and legumes growing in the soil covering four coal fly ash landfill sites in Central New York were sampled and analyzed for 20 elements. Selenium, boron and molybdenum were most consistently higher in concentration in these crops than the corresponding control plants sampled from adjacent upwind locations. Legumes absorbed greater amounts of these elements than grasses probably owing to their deep‐rooted penetration of the fly ash layer below. Factors affecting the extent of absorption of these elements by the crops and the agricultural significance as related to grazing farm animals are discussed.

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Fluorometric Analysis of Selenium in Plants

Cited by 73 publications

References 0 publications

Variation in the Selenium Content of Individual Brazil Nuts

Variation in the Selenium Content of Individual Brazil Nuts

Selenium and glutathione peroxidase in blood and tissues and growth and feed efficiency in young bulls at different dietary selenium levels

Elemental Analysis of Grasses and Legumes Growing on Soil Covering Coal Fly Ash Landfill Sites

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