Fluoroimmunoassay of Alphafetoprotein (AFP) in Amniotic Fluid

Sinosich, Michael J.; Chard, T.

doi:10.1177/000456327901600188

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Heterogeneous Assays1

Indirect Immunofluorescence1

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1981

1999

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Cited by 5 publications

(2 citation statements)

References 4 publications

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“…For the reasons described previously, the performance of fluorescent assays involving organic markers does not exceed that of the corresponding radioimmuno assays (EKEKE et al 1979;KOBAYASHI et al 1982;KAPLAN et al 1981;TSAyet al 1980;SINOSICH and CHARD 1979;KARNES et al 1981). In contrast, the use of rare earth chelates gives very good results.…”

Section: Heterogeneous Assaysmentioning

confidence: 96%

Radioiodination and Other Labeling Techniques

Grassi¹,

Maclouf²,

Pradelles³

1987

Radioimmunoassay in Basic and Clinical Pharmacology

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Section: Heterogeneous Assaysmentioning

confidence: 96%

Radioiodination and Other Labeling Techniques

Grassi¹,

Maclouf²,

Pradelles³

1987

Radioimmunoassay in Basic and Clinical Pharmacology

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“…Primary antibody, rabbit anti-guinea pig heparin binding placental protein (HBPP), was used at a 1/50 dilution. Secondary antibody (2nd Ab), swine antirabbit immunoglobulins (Dakopatts, Santa Barbara, CAI, was conjugated to fluorescein isothiocyanate (FITC; Sigma) in alkaline phosphate buffer (pH 8.6; Sinosich and Chard, 1979). The 2nd Ab-FITC conjugate was used at 1/200 dilution.…”

Section: Indirect Immunofluorescencementioning

confidence: 99%

Immunocytochemical studies of hamster oocyte activation

Sinosich

Lanzendorf

Hodgen

1990

Molecular Reproduction Devel

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By indirect immunofluorescence, using rabbit anti-heparin-binding placental protein (HBPP) antiserum, we studied HBPP expression by physiologically and non-physiologically (microsurgically) activated hamster gametes. Whereas mature gametes (sperm, metaphase II oocytes) were negative, in vivo conceived preimplantation embryos, from pronuclear to two- and four-cell stages, were HBPP positive. No HBPP was demonstrated in the zona pellucida, but HBPP-dependent immunofluorescence was localized in the perivitelline space. Oocytes incubated with hyaluronidase demonstrated variable responses from negative to positive. (Diluent or sperm) microinjected oocytes were all activated and HBPP positive within 4 h after stimulation. Thus neither activation by microinjection nor HBPP expression required paternal gametes. These kinetics suggest that HBPP may be a cortical granule secretogogue which can be applied to monitor oocyte responses during in vitro manipulations.

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Immunoassays Employing Reactants Labelled with a Fluorophore

Landon¹,

Kamel²

1981

Immunoassays for the 80s

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Fluoroimmunoassay of Alphafetoprotein (AFP) in Amniotic Fluid

Cited by 5 publications

References 4 publications

Radioiodination and Other Labeling Techniques

Radioiodination and Other Labeling Techniques

Immunocytochemical studies of hamster oocyte activation

Immunoassays Employing Reactants Labelled with a Fluorophore

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