Fluorogenic Probing of Specific Recognitions between Sugar Ligands and Glycoprotein Receptors on Cancer Cells by an Economic Graphene Nanocomposite

Abstract

Economical nanocomposites based on π-stacking of N-acetyl glycosyl rhodamine B to graphene oxide (GO) are simply prepared. These "sweet" GO-materials are proven to be admirable for the fluorogenic recognition of specific intercellular sugar-based ligand-glycoprotein receptor interactions of interest.

Toehold-mediated nonenzymatic amplification circuit on graphene oxide fluorescence switching platform for sensitive and homogeneous microRNA detection

“…It is still ambiguous whether dsDNA containing several singlestranded toeholds cannot be effectively adsorbed by GO [45,46], or whether it can be anchored onto the GO surface by its single stranded toeholds (as shown in Fig. 3A) [47,48].…”

“Clicked” galactosyl anthraquinone on graphene electrodes for the label-free impedance detection of live cancer cells

“…Hep-G2 cells with the expression of asialoglycoprotein receptors (ASGPr) that recognize selectively galactose [27][28][29][30] were incubated with the electrodes at two different cell concentrations (500,000 and 10,000 cells mL -1 ). As a result, we observed an evident R ct increase in the presence of Hep-G2 in a concentration-dependent manner ( Fig.…”

“…we also used an established cell line with largely reduced ASGPr expression level (sh-ASGPr) [27][28][29][30]. To our delight, reduction of the receptor expression led to a largely decreased impedance increment (Fig.…”

Anthraquinonyl glycoside facilitates the standardization of graphene electrodes for the impedance detection of lectins

“…In the Raman spectra, the intensity ratio of the D band (1355 cm −1 ) to the G band (1600 cm −1 ) of the ZBW1 - nG complex increased (0.92, Figure 3a) comparing to the bare nG (0.86, Figure 3b). This suggests an increase in sp 2 -hybridization of the complex probably because of the stacking of the aromatic ZBW1 to the surface of graphene [4,5,20,21]. In the meanwhile, peaks characteristic of the stacking of AG to nG were observed in the UV–vis (red shift from 388 nm [orange] to 395 nm [violet] Figure 3c) and FTIR ( ν̃ = 2350 cm −1 , Additional file 1: Figure S4) spectra of the ZBW1 - nG complex.…”