Fluorogenic probe triggered by reduction for nucleic acids sensing

Abstract

A reduction-triggered fluorescence probe with a new fluorogenic compound derivatized from rhodamine 110 was developed for sensing oligonucleotides. The chemistry to activate the compound involves the reaction between the azide group of rhodamine derivatives and reducing reagents, with the fluorescence signal appearing after reduction of the azide group. The reaction proceeds under biological conditions to produce fluorescence signal within 10-20 min in the presence of target DNA or RNA. The probes were successfully applied to the detection of oligonucleotides in solution and endogenous RNA in bacterial cells.

Recent advances in living cell nucleic acid probes based on nanomaterials for early cancer diagnosis

“…Organic dyes are fluorophores that emit in the ultraviolet–visible (UV–vis) and near-infrared (NIR) regions, and they are small, water-soluble, and easy to use for biological coupling [118] . Common types include the BODIPY compounds [119] , fluorescein [120] , cyanine family [121] , and rhodamine family [122] . Among them, 4-(4′-dimethylaminophenylazo) benzoic acid (Dabcyl) and the black hole quenching agent (BHQ) are often used as quenching agents.…”