Fluorine-18 Fluorodeoxyglucose Uptake in Hepatocellular Carcinoma: Correlation with Glucose Transporters and p53 Expression

Abstract

Introduction: Hepatocellular Carcinoma (HCC) is one of most lethal cancers worldwide. The prognosis is very poor and therapeutic options are limited. The aim of this study was to determine the correlation of the [ 18 F]FDG uptake profile of three HCC cell lines with p53 and glucose transporters (GLUTs) 1, 2, 3, 5 and 12 expression and with the glucose level present in the cell culture medium. Methods: Cell lines used are HepG2 (wp53), HuH7 (overexpress p53) and Hep3B2.1-7 (p53null). An immunocytochemical analysis was performed to evaluate p53 expression. Through uptake studies were analyzed the

Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

“…Quantification of fluorescence was performed using ImageQuant 5.0 (Molecular Dynamics) [26]. Hep3B2.1-7 cell line has a null P53, as already proved by our team, reason why we not provided P53 results for this cell line [28,29]. [30,31].…”

CMA down-regulates p53 expression through degradation of HMGB1 protein to inhibit irradiation-triggered apoptosis in hepatocellular carcinoma

“…Besides the CMA pathway, there are varieties of cellular processes that determine the success of treatment, including p53 protein[26]. p53 is a transcription factor associated with more than 50% of human tumors and a key molecule involved in response to cell apoptosis in radiotherapy[27-29]. In our study, apoptosis was much lower on irradiation in Hep3B cells (p53-/-) than in HepG2 cells.…”

Targeting hepatocellular carcinoma: what did we discover so far?

“…It is known that among the most common molecular changes observed in HCC are mutations in the TP53 gene and in the P53 protein. 31,32,35,36 Under normal conditions, P53 is present in the cells in low concentrations but transcriptional activation of this protein may occur in response to various stimuli including DNA damages, hypoxia or deregulated cell cycle progression. P53 protein has several important biological functions including the maintenance of the cell cycle, repair of DNA damage, the balance between cell proliferation and apoptosis, the response to cellular stress and inhibition of angiogenesis.…”