Fluorination Enhances NIR‐II Fluorescence of Polymer Dots for Quantitative Brain Tumor Imaging

Liu, Ye; Liu, Jinfeng; Chen, Dandan; Wang, Xiaosha; Zhang, Zhe; Yang, Yicheng; Jiang, Lihui; Qi, Weizhi; Ye, Ziyuan; He, Shuqing; Liu, Quanying; Xi, Lei; Zou, Yingping; Wu, Changfeng

doi:10.1002/ange.202007886

Cited by 17 publications

(7 citation statements)

References 62 publications

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“…Upconversion nanoparticles are developed based on their deep tissue penetration and weak background interference, yet complex mechanisms associated with a nonlinear photon upconversion impede quantification by means of fluorescence intensity. Semiconducting polymer dots (Pdots) have received considerable attention in biology and medicine. − Various Pdots have been demonstrated in applications such as optical imaging, photoacoustic imaging, biosensing, phototherapy, and drug delivery. − Pdots exhibit stable fluorescence intensity in a physiological environment comprising biologically relevant ions and ROS . The Pdots also remain highly dispersed without a sign of aggregation in a biological medium.…”

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confidence: 99%

Measuring Cellular Uptake of Polymer Dots for Quantitative Imaging and Photodynamic Therapy

et al. 2021

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There is a great deal of interest in the development of nanoparticles for biomedicine. The question of how many nanoparticles are taken up by cells is important for biomedical applications. Here, we describe a fluorescence method for the quantitative measurement of the cellular uptake of polymer dots (Pdots) and a further estimation of intracellular Pdots photosensitizer for fluorescence imaging and photodynamic therapy. The approach relies on the high brightness, excellent stability, minimal aggregation quenching, and metalloporphyrin doping properties of the Pdots. We correlated the single-cell fluorescence brightness obtained from fluorescence spectrometry, confocal microscopy, and flow cytometry with the number of endocytosed Pdots, which was validated by inductively coupled plasma mass spectrometry. Our results indicated that, on average, ∼1.3 million Pdots were taken up by single cells that were incubated for 4 h with arginine 8-Pdots (40 μg/mL, ∼20 nm diameter). The absolute number of endocytosed Pdots of individual cells could be estimated from confocal microscopy by comparing the single-cell brightness with the average intensity. Furthermore, we investigated the cell viability as a result of an intracellular Pdots photosensitizer, from which the half maximal inhibitory concentration was determined to be ∼7.2 × 105 Pdots per cell under the light dose of 60 J/cm2. This study provides an effective method for quantifying endocytosed Pdots, which can be extended to investigate the cellular uptake of various conjugated polymer carriers in biomedicine.

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Measuring Cellular Uptake of Polymer Dots for Quantitative Imaging and Photodynamic Therapy

et al. 2021

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“…As a result, the development of a multifunctional nanoplatform capable of crossing the BBB and targeting gliomas is critical for the high-sensitivity detection and ablation of cancer cells. Although Pdots-based FL imaging for glioma detection was carried out [110,111,115], as exogenous substances, they are easily recognized by the immune system and quickly cleaned by the liver and kidney [110]. Additionally, pure Pdots have difficulties in crossing BBB and specifically targeting brain tumor tissue.…”

Section: Pdots For Glioma Imagingmentioning

confidence: 99%

A review of biomodified or biomimetic polymer dots for targeted fluorescent imaging and disease treatments

Guo

Chen

et al. 2023

iRADIOLOGY

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Due to their inherent tunable spectrum, high brightness, excellent biostability and biocompatibility, and functionalization of surfaces, semiconducting polymer dots (Pdots) are now playing an essential role in fluorescent (FL) imaging and disease treatment through bioconjugation with peptides or biomimetic materials. In particular, biomimetic Pdots exhibit their capability in targeted imaging of lesion and increased efficacy for targeting disease treatment. This review will inspect the recent advances in the design and functionalization strategies of biomodified and biomimetic Pdots for enhanced disease detection and therapy. More importantly, the application of these two modifications in targeted FL imaging and cancer treatment is to be addressed in detail. Meanwhile, the main challenges and prospects of biomimetic and biomodified Pdots are to be discussed, which will pave a new avenue for improved disease detection and imaging‐guided treatment.

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“…On the other hand, halogenation is a feasible strategy to influence the photophysical properties of organic dyes, such as photostability, molecular packing, emission efficiency, and so on [38–48] . Some studies have also been conducted and reported to investigate the effects of halogen substitutions on AIEgens [49–55] .…”

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confidence: 99%

Effects of Halogenation on Quinoline‐Malononitrile‐based AIEgens: Photophysical Properties Investigation and Wash‐Free Imaging

Feng

Meng

Niu

2023

Chemistry An Asian Journal

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Developing halogen-functionalized fluorescent dyes with intriguing photophysical properties, including enhanced photostability, is particularly important for bioimaging. In this work, we synthesized two new halogenfunctionalized aggregation-induced emission (AIE)-active molecules, DQMF-OH and DQMCl-OH, based on the quinoline-malononitrile chromophore. The halogen effect on the photophysical characteristics was detailedly studied by absorption and fluorescence spectroscopy, density functional theory calculations, and crystal structures. Compared with non-halogen substituted AIE luminogen (AIEgen) DQM-OH, the halogen substituted DQMF-OH and DQMCl-OH exhibited red-shifted absorptions and emissions in the solution and solid state. In addition, DQMF-OH and DQMCl-OH also possessed enhanced fluorescence toward viscosity changes. These AIEgens served as remarkable imaging tools for cell tracking in a wash-free manner. Furthermore, DQMF-OH and DQMCl-OH showed much more excellent photobleaching resistance than DQM-OH. Our work sheds new light on developing fluorescent halogenated dyes with enhanced photophysical performances for biological applications.

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Fluorination Enhances NIR‐II Fluorescence of Polymer Dots for Quantitative Brain Tumor Imaging

Cited by 17 publications

References 62 publications

Measuring Cellular Uptake of Polymer Dots for Quantitative Imaging and Photodynamic Therapy

Measuring Cellular Uptake of Polymer Dots for Quantitative Imaging and Photodynamic Therapy

A review of biomodified or biomimetic polymer dots for targeted fluorescent imaging and disease treatments

Effects of Halogenation on Quinoline‐Malononitrile‐based AIEgens: Photophysical Properties Investigation and Wash‐Free Imaging

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