Fluorinated Tags to Study Protein Conformation and Interactions Using 19F NMR
George S. M. Hanson,
Christopher R. Coxon
Abstract:The incorporation of fluorine atoms into a biomacromolecule provides a background‐free and environmentally sensitive reporter of structure, conformation and interactions using 19F NMR. There are several methods to introduce the 19F reporter – either by synthetic incorporation via solid phase peptide synthesis; by suppressing the incorporation or biosynthesis of a natural amino acid and supplementing the growth media with a fluorinated counterpart during protein expression; and by genetic code expansion to add … Show more
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