Fluorimetric determination of the fungicide benomyl after solvolysis

Abstract

Abstract.A simple spectrofluorimetric method for the determination of the fungicide benomyl in grapes is described. Benomyl is rapidly solvolysed in organic solvents to give the highly fluorescent metabolite carbendazim. Solvent effects on the spectral shape and their influence on analytical performance are studied. DMF was found to be the best solvent. The method is sensitive to 12 ng/ml benomyl, and has an RSD at the 0.5 #g/ml level of 2.2%. The method is suitable for determining benomyl in spiked grapes, with recoveries in the range 97 to 104~.

Liquid chromatographic determination of benomyl in water samples after dispersive liquid–liquid microextraction

“…According to Scheme 1, BN is converted into CB in organic solvents via solvolysis reaction [3]. We therefore decided to combine this reaction with DLLME for conversion of BN during the microextraction process followed by liquid chromatographic determination of BN as CB.…”

“…There are various analytical methods for the analysis of BN in different samples: Liquid chromatography [1,2], fluorimetry [3,4], fluorescence polarization [5], and modulated anisotropy fluorescence [6] have been reported in the literature. However, in order to determine trace amounts of the fungicide, development of novel methods with higher sensitivities, precision, and accuracy is necessary.…”

Kinetic stabilization of microtubule dynamic instability by benomyl increases the nuclear transport of p53

“…Benomyl was shown not to inhibit the binding of colchicine to mammalian tubulin and it was also found to bind to the tubulin-colchicine complex [10]. Benomyl is quite unstable and it decomposes in aqueous solutions into more stable compounds like carbendazim, and N,N 0 -dibutyl urea [11,12]. Carbendazim is the functionally active metabolite, which is believed to have similar actions as benomyl [1,6,13,14].…”

“…Carbendazim is the functionally active metabolite, which is believed to have similar actions as benomyl [1,6,13,14]. The conversion of benomyl to carbendazim is slow in aqueous environment and it could take several days [11,12].…”

Spectrofluorimetric Analysis of the Fungicide Carbendazim and Its Metabolite 2-Aminobenzimidazole in Natural Water

“…These limits correspond to concentrations about 40 times lower than that of MBC and 10 times lower than that of 2-AB. These results can be explained by the fact that benomyl is a compound that is metabolized to MBC during ordinary analytical procedures in organic solvents and water [2] [16] [17]. The 2-AB is also a metabolite of MBC, and the three benzimidazoles emit at the same wavelength region.…”