Fluorimetric Determination of Peroxynitrite Based on an Enzymatic Reaction

Cao, Qi; Qian, Zhou; Cai, Ru Xiu; Liu, Fei

doi:10.2116/analsci.21.445

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…The concentration of O 2 − and H 2 O 2 was detected and referred to a standard curve as in Reverberi et al [ 3 ]. Peroxynitrite (ONOO − was assayed as described in Cao et al [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

Menadione-Induced Oxidative Stress Re-Shapes the Oxylipin Profile of Aspergillus flavus and Its Lifestyle

Zaccaria

Ludovici

Sanzani

et al. 2015

Toxins

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Aspergillus flavus is an efficient producer of mycotoxins, particularly aflatoxin B1, probably the most hepatocarcinogenic naturally-occurring compound. Although the inducing agents of toxin synthesis are not unanimously identified, there is evidence that oxidative stress is one of the main actors in play. In our study, we use menadione, a quinone extensively implemented in studies on ROS response in animal cells, for causing stress to A. flavus. For uncovering the molecular determinants that drive A. flavus in challenging oxidative stress conditions, we have evaluated a wide spectrum of several different parameters, ranging from metabolic (ROS and oxylipin profile) to transcriptional analysis (RNA-seq). There emerges a scenario in which A. flavus activates several metabolic processes under oxidative stress conditions for limiting the ROS-associated detrimental effects, as well as for triggering adaptive and escape strategies.

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“…The concentration of O 2 − and H 2 O 2 was detected and referred to a standard curve as in Reverberi et al [ 3 ]. Peroxynitrite (ONOO − was assayed as described in Cao et al [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

Menadione-Induced Oxidative Stress Re-Shapes the Oxylipin Profile of Aspergillus flavus and Its Lifestyle

Zaccaria

Ludovici

Sanzani

et al. 2015

Toxins

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show abstract

“…Another novel spectrofluorimetric method for the determination of peroxynitrite uses hemoglobin (Hb) as a catalyst and thiamine (Cao et al 2005) or l-tyrosine as the substrate (Liang et al 2005). Hb is used as the mimetic enzyme for substituting horseradish peroxidase for two reasons: (1) for the purpose of the mildness of reaction conditions and the minimization of cost and (2) Hb is a kind of nature protein in human body and it is much likely to coexist with peroxynitrite in cell or organism (Liang et al 2005).…”

Section: Measurement Of Other Reactive Oxygen/nitrogen Speciesmentioning

confidence: 99%

Review of Methods to Determine Antioxidant Capacities

2009

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Antioxidant capacity is related with compounds capable of protecting a biological system against the potentially harmful effect of processes or reactions involving reactive oxygen and nitrogen species (ROS and RNS). These protective effects of antioxidants have received increasing attention within biological, medical, nutritional, and agrochemical fields and resulted in the requirement of simple, convenient, and reliable antioxidant capacity determination methods. Many methods which differ from each other in terms of reaction mechanisms, oxidant and target/probe species, reaction conditions, and expression of results have been developed and tested in the literature. In this review, the methods most widely used for the determination of antioxidant capacity are evaluated, presenting the general principals, recent applications, and their strengths and limitations. Analysis conditions, substrate, and antioxidant concentration should simulate real food or biological systems as much as possible when selecting the antioxidant capacity method. The total antioxidant capacity value should include methods applicable to both lipophilic and hydrophilic antioxidants, with regards the similarity and differences of both hydrogen atom transfer and electron transfer mechanism. The methods including various ROS/RNS also have to be designed to comprehensively evaluate the antioxidant capacity of a sample.

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“…Most recently published detection methods for peroxynitrite, if not all, are fluorescent (Cao et al, 2005;Du and Guo, 2008;Huang et al, 2007;Martin-Romero et al, 2004;Panizzi et al, 2009;Sun et al, 2009;Zheng et al, 2006;Yang et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Nanostructured poly(3,4-ethylenedioxythiophene)–metalloporphyrin films: Improved catalytic detection of peroxynitrite

Peteu

Peiris

Gebremichael

et al. 2010

Biosensors and Bioelectronics

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We investigated in this paper the sensing performance of inherently conductive polymer, poly(3,4-ethylenedioxythiophene) (PEDOT), functionalized with hemin (iron protoporphyrin) as an electrocatalytic reporter. The sensing platform is prepared by electrodeposition of a composite film of hemin-PEDOT on a 30-microm diameter carbon fiber electrode (CFE). The polymerized films were characterized by field emission scanning electron microscopy (FESEM), which pointed to nanostructured films with tortuous pores. The electrocatalytic oxidation of peroxynitrite was characterized by cyclic voltammetry as well as other electrochemical methods. The catalytic current is proportional to the analyte's concentration. Optimized hemin-PEDOT modified CFEs were utilized for the first time to detect ONO2(-), with a response time down to 5 s and a limit of detection as low as 200 nM as evidenced by amperometry. Our hemin-PEDOT modified CFEs have a sensitivity of 13 nA/microM, ca.130 times higher than the bare CFE. More work is underway using other metalloporphyrins as electrocalalysts to improve the detection limit, the selectivity, and to further miniaturize these hemin-PEDOT modified electrodes.

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Fluorimetric Determination of Peroxynitrite Based on an Enzymatic Reaction

Cited by 6 publications

References 13 publications

Menadione-Induced Oxidative Stress Re-Shapes the Oxylipin Profile of Aspergillus flavus and Its Lifestyle

Menadione-Induced Oxidative Stress Re-Shapes the Oxylipin Profile of Aspergillus flavus and Its Lifestyle

Review of Methods to Determine Antioxidant Capacities

Nanostructured poly(3,4-ethylenedioxythiophene)–metalloporphyrin films: Improved catalytic detection of peroxynitrite

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