Fluorimetric chemodosimeter for the detection of capsaicinoids in food matrices

Servarayan, Karthika Lakshmi; Sundaram, Ellairaja; Aravind, M.; Lachathipathi, Prakash; Ashokkumar, Balasubramaniem; Vasantha, Vairathevar Sivasamy

doi:10.1016/j.foodchem.2023.135843

Cited by 3 publications

(2 citation statements)

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“…Ions and chemicals (Na + , K + , Mg 2+ , Ca 2+ , Fe 2+ , Cl − , D-(+)-glucose, and citric acid) were selected as interfering compounds due to their ubiquity. 46 Consequently, they exhibited negligible interference with Fe 0.7 /NC@PSS-ELISA for CAP detection. Overall, Fe 0.7 /NC@PSS-ELISA demonstrated high sensitivity and selectivity for CAP, making it suitable for practical applications.…”

Section: ■ Results and Discussionmentioning

confidence: 96%

“…An interference study was conducted to evaluate the selectivity of Fe 0.7 /NC@PSS-ELISA (Figure D). Ions and chemicals (Na + , K + , Mg 2+ , Ca 2+ , Fe 2+ , Cl – , d -(+)-glucose, and citric acid) were selected as interfering compounds due to their ubiquity . Consequently, they exhibited negligible interference with Fe 0.7 /NC@PSS-ELISA for CAP detection.…”

Section: Resultsmentioning

confidence: 99%

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Novel Immunosensor Based on Metal Single-Atom Nanozymes with Enhanced Oxidase-Like Activity for Capsaicin Analysis in Spicy Food

Yu,

Jia,

Xing

et al. 2024

J. Agric. Food Chem.

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Capsaicin (CAP) is a primary indicator for assessing the level of pungency. Herein, iron-based single-atom nanozymes (SAzymes) (Fe/NC) with exceptional oxidase-like activity were used to construct an immunosensor for CAP analysis. Fe/NC could imitate oxidase actions by transforming O 2 to • O 2 − radicals in the absence of hydrogen peroxide (H 2 O 2 ), which could avoid complex operations and unstable results. By regulating the Fe atom loads, an optimal Fe 0.7 /NC atom usage rate could improve the catalytic activity (Michaelis−Menten constant (K m ) = 0.09 mM). Fe 0.7 /NC was integrated with goat antimouse IgG by facile mix incubation to develop a competitive enzyme-linked immunosorbent assay (ELISA). Our Fe 0.7 /NC immunosensing platform is anticipated to outperform the conventional ELISA in terms of stability and shelf life. The proposed immunosensor provided color responses across 0.01−1000 ng/mL CAP concentrations, with a detection limit of 0.046 ng/mL. Fe/NC may have potential as nanozymes for CAP detection in spicy foods, with promising applications in food biosensing.

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Section: ■ Results and Discussionmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%