Fluoride-Resistant Acid Phosphatase (FRAP)-Positive Primary Afferent Central Terminals in the Mouse Substantia Gelatinosa.

Hiura, Akio; Ishizuka, Hiroshi; Ohta, Mika

doi:10.1267/ahc.28.501

Cited by 4 publications

(1 citation statement)

References 26 publications

(31 reference statements)

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“…1986; Hammond and Ruda, 1991, Holzer, 1991, Hiura and Ishizuka, 1994Hiura et al, 1995). However, quantitative estimates of the reduction in extent or width of these laminae has received less attention.…”

Section: Terminal Labelling In Vc and Cmentioning

confidence: 99%

Effect of neonatal capsaicin and infraorbital nerve section on whisker-related patterns in the rat trigeminal nucleus

Waite

Permentier

1997

J. Comp. Neurol.

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In the present study, we investigated the effect of neonatally administered capsaicin on whisker-related pattern formation in the rat trigeminal complex. Both normal whisker-related patterns of barrelettes and the modified patterns seen after neonatal section of the infraorbital nerve were assessed. Capsaicin caused no change in the pattern or size of cytochrome oxidase (CO) barrelettes in the principal trigeminal nucleus (Vp) or trigeminal nucleus interpolaris (Vi) or caudalis (Vc). Injections of horseradish peroxidase (HRP) or wheatgerm agglutinin conjugated to HRP (WGA-HRP) into the posteroorbital (PO) whisker follicle in vehicle-treated animals showed that WGA labelled a larger number of trigeminal ganglion cells than HRP (203 +/- 23; cf. 158 +/- 19), with an increased labelling of small-diameter neurons (HRP: 25.9 +/- 7.7 microm; WGA: 23.2 +/- 7.2 pm). Capsaicin caused a loss of smaller diameter cells but had no effect on the location, cross-sectional area, or rostrocaudal extent of the transganglionically labelled HRP terminations in Vp, Vi, Vc, and cervical dorsal horn. WGA-HRP labelling revealed similar, but less dense, central terminal areas as HRP and an additional area of superficial terminals in the caudal medulla; these were also unaffected by capsaicin treatment. After infraorbital nerve section, CO patches and transganglionically labelled afferent terminations, corresponding to innervated nonmystacial whiskers, were approximately doubled in size. Capsaicin had no effect on the increased size of these spared whisker patches or their afferent terminal areas. These results suggest that barrelette formation is not dependent on unmyelinated afferents and that the changes in response properties seen after capsaicin, such as increased receptive fields, reflect functional changes rather than anatomical expansion of afferent terminal areas.

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Section: Terminal Labelling In Vc and Cmentioning

confidence: 99%

Effect of neonatal capsaicin and infraorbital nerve section on whisker-related patterns in the rat trigeminal nucleus

Waite

Permentier

1997

J. Comp. Neurol.

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Neuroanatomical Effects of Capsaicin on the Primary Afferent Neurons.

Hiura

2000

Archives of Histology and Cytology

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Studies by N. JANCSO and his associates in the 1970's established that capsaicin in paprika exerts selective damage on nociceptive primary sensory neurons. The physiological and pharmacological aspects of capsaicin's effect have been repeatedly reviewed, but no report seems available concerning the neuroanatomical changes caused by capsaicin. This paper first reviews the neuroanatomical aspect of the lesion caused by capsaicin. Special attention is paid to quantitative estimations made by our group and others on the loss of dorsal root ganglion (DRG) cells, dorsal root nerve fibers, the saphenous nerve, chorda tympani nerve, and pulp nerves after neonatal treatment with capsaicin. The degenerating process of DRG cells induced by capsaicin is discussed with respect to necrosis and apoptosis. The capsaicin receptors found recently are concisely introduced with reference to their action. A discrepancy between a marked loss of dorsal root C-fibers and an unexpectedly intact response to noxious heat in mice treated with capsaicin at neonate is discussed, and attension is given to nerves sprouting from capsaicin-resistant DRG cells in the superficial dorsal horn. In addition, the architecture of the synapses between the central endings of the capsaicin-sensitive primary afferent neurons and the intrinsic inhibitory interneurons is described and its possible significance considered in terms of the transmission of nociceptive information.

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Fluoride-resistant Acid Phosphatase (FRAP)-positive Afferent Terminals Make Synaptic Contact with Interneuronal Soma in the Substantia Gelatinosa of the Mouse Spinal Dorsal Horn

Hiura

Nasu

Kuwahara

et al. 1997

Okajimas Folia Anatomica Japonica

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Summary: Fluoride-resistant acid phosphatase (FRAP)-reactive terminals making contact with interneuronal soma are found in the substantia gelatinosa of the mouse spinal dorsal horn. About one half of the interneuronal somata receive FRAP-positive boutons. By electron microscopy, these FRAP-positive terminals appear small, dark, slender, roundish, cap-like, ellipsoid or sinuous and electron-dense, scalloped (fan-like) contours with dear spherical synaptic vesicles of variable size, some large dense-core vesides and mitochondria. All these features are very similar to those of capsaicinsensitive terminals. Thus they are considered to be nodceptive primary afferent endings. Therefore, some of the FRAPpositive terminals are suggested to have a modulatory role in the nociceptive circuit in the substantia gelatinosa.

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Fluoride-Resistant Acid Phosphatase (FRAP)-Positive Primary Afferent Central Terminals in the Mouse Substantia Gelatinosa.

Cited by 4 publications

References 26 publications

Effect of neonatal capsaicin and infraorbital nerve section on whisker-related patterns in the rat trigeminal nucleus

Effect of neonatal capsaicin and infraorbital nerve section on whisker-related patterns in the rat trigeminal nucleus

Neuroanatomical Effects of Capsaicin on the Primary Afferent Neurons.

Fluoride-resistant Acid Phosphatase (FRAP)-positive Afferent Terminals Make Synaptic Contact with Interneuronal Soma in the Substantia Gelatinosa of the Mouse Spinal Dorsal Horn

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