Fluoride or/and aluminum induced toxicity in guinea pig teeth with the low expression of dentine phosphoprotein

Abstract

This study investigated the damage and expression of dentine phosphoprotein (DPP) in guinea pig teeth by the administration of fluoride (F) or/and aluminum (Al). Fifty-two guinea pigs were divided randomly into four groups (control, F, Al, and F+Al). F (150 mg NaF/L) or/and Al (300 mg AlCl /L) were added in their drinking water for 90 days. The levels of F ion, dentine sialophosphoprotein (DSPP) gene, and DPP protein in incisor and molar were determined, respectively. The results showed that the concentrations of F ion in F and F+Al groups were increased significantly. F induced the mottled enamel and irregular abrasion of teeth, which might occur as a consequence of depressed DSPP mRNA and DPP protein expression. Both the gene and protein expressions showed obvious decrease induced by Al, especially by F. There were no synergistic effects between F and Al, instead, Al inhibited the toxicity of F.

Biomimicry revolutionizing dentistry: A review

“…Human Dentine Phosphoprotein (DPP): Found in dentine, DPP captures calcium and phosphate ions, influencing crystal growth and orientation. Researchers are exploring peptides containing DPP's key sequences to regulate enamel regeneration [36,37] . Dendrimers: Versatile artificial polymers, are being explored for enamel regeneration.…”

Cell-Free Biomimetic Mineralization Strategies to Regenerate the Enamel Microstructure

“…A constituent of the extracellular matrix of dentine, DPP captures free Ca 2+ and PO 4 3− ions. DPP is involved in mineralization of the entire dentine layer and in bone calcification [60]. DPP contains numerous, repetitive aspartate-serine-serine (DSS) nucleotide sequences that function in inhibiting calcium phosphate dissolution and controlling crystal growth during dentine mineralization [29].…”