Fluoride induced testicular toxicities in adult Wistar rats

Pal, Priyankar; Mukhopadhyay, P. K.

doi:10.1080/15376516.2021.1891489

Cited by 10 publications

(3 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The main contributor to fluoride‐induced testes toxicity has been identified as oxidative stress. The pathogenesis of fluoride‐induced testicular damage is linked to a number of factors, one of which is the formation of ROS [7,20] . Fluoride may impair mitochondrial function, impede cellular respiration, and increase free radical generation in the mitochondria, leading to testes failure.…”

Section: Resultsmentioning

confidence: 99%

“…The pathogenesis of fluoride-induced testicular damage is linked to a number of factors, one of which is the formation of ROS. [7,20] Fluoride may impair mitochondrial function, impede cellular respiration, and increase free radical generation in the mitochondria, leading to testes failure. In our study, NaF caused testes oxidative damage, as demonstrated by lower antioxidant enzyme (Cat, SOD and GPx) levels in the NaF-treated group.…”

Section: Ameliorative Effects Of Hes On Naf-induced Er Stress Markersmentioning

confidence: 99%

See 1 more Smart Citation

Contribution of Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress and Autophagy Pathways to the Ameliorative Effects of Hesperidin in NaF‐Induced Testicular Toxicity

Kızıl¹,

Gür²,

Ayna³

et al. 2023

Chemistry & Biodiversity

View full text Add to dashboard Cite

The ameliorative effects of hesperidin (HES) on the toxicities created by sodium fluoride (NaF) in the testes tissue of rats were studied via oxidative stress, apoptosis and endoplasmic reticulum (ER) stress pathways. The animals were divided into five distinct groups (7 rats in each group). Group 1 was control group, group 2 received NaF-only (600 ppm), group 3 received HES-only (200 mg/kg bw); group 4 received NaF (600 ppm) + HES (100 mg/kg bw) and group 5 received NaF (600 ppm) + HES (200 mg/kg bw) for 14 days. NaF-induced testes tissue damage by reducing activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) and levels of glutathione (GSH), and increasing lipid peroxidation levels. NaF treatment significantly downregulated the mRNA levels of SOD1, CAT and GPx. NaF supplementation caused apoptosis in the testes by upregulating p53, NFkB, caspase-3, caspase-6, caspase-9, and Bax and downregulating Bcl-2. Furthermore, NaF caused ER stress via increasing mRNA transcript levels of PERK, IRE1, ATF-6 and GRP78. NaF treatment led to autophagy via upregulation of Beclin1, LC3A, LC3B and AKT2. In testes tissue, however, co-treatment with HES at doses of 100 and 200 mg/kg significantly reduced oxidative stress, apoptosis, autophagy and ER stress. Overall, the findings of this study suggest that HES may help to reduce testes damage caused by NaF toxicity.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Ameliorative Effects Of Hes On Naf-induced Er Stress Markersmentioning

confidence: 99%

Contribution of Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress and Autophagy Pathways to the Ameliorative Effects of Hesperidin in NaF‐Induced Testicular Toxicity

Kızıl¹,

Gür²,

Ayna³

et al. 2023

Chemistry & Biodiversity

View full text Add to dashboard Cite

show abstract

“…This reaction is crucial for the regulation of the antioxidant defense system. To assess SOD activity, we followed the protocol established by Pal and Mukhopadhyay [28]. Testicular tissue was homogenized in 1 mL of phosphate buffer (pH 7.4) and then centrifuged at 10,000 ×g for 10 minutes at 4 °C.…”

Section: Determination Of Antioxidant Levels Via Superoxide Dismutase...mentioning

confidence: 99%

Protective effect of platelet-rich plasma against structural and functional changes of the adult rat testis in carbimazole-induced hypothyroidism

Bordbar,

Sattar-Shamsabadi,

Dehghani

et al. 2024

Clin Exp Reprod Med

View full text Add to dashboard Cite

Objective: Hypothyroidism (HT) influences spermatogenesis and is associated with male infertility. Platelet-rich plasma (PRP), a biological product rich in growth factors, promotes tissue repair. In this study, the likely protective effects of PRP on testicular tissue damage in carbimazole (CBZ)-induced HT were evaluated. Methods: Forty male rats were divided into four groups. HT was induced by administering CBZ (1.35 mg/kg orally, for 45 days). Two doses of PRP (40 μL each, locally injected into the testis on days 15 and 30) were also given. After 45 days, blood samples were taken from the heart to measure triiodothyronine (T3), thyroxine (T4), and testosterone levels, and semen analysis was performed. For stereological assessment, the left testis was removed, fixed, embedded, sectioned, and stained with hematoxylin and eosin. The right testis was excised to evaluate antioxidant levels.Results: CBZ was demonstrated to induce HT, characterized by significant reductions in T3 and T4. HT was associated with decreased testicular weight, impaired sperm parameters, reduced testosterone concentration, diminished antioxidant activity, reduced volumes of testicular components, and lower total numbers of testicular cells of various types. When HT samples were treated with PRP, improvement was observed for all of these changes. This protective effect could be attributed to the growth factors present in PRP.Conclusion: PRP appears to prevent the structural changes in the testes and the deterioration in sperm quality caused by CBZ-induced HT. This protective effect is likely due to mitigation of oxidative damage and elevation of testosterone levels.

show abstract

Exposure to Fluoride From in Utero to Puberty Alters Gonadal Structure and Steroid Hormone Expression in Offspring Rats

Sun

et al. 2022

Biol Trace Elem Res

View full text Add to dashboard Cite

Fluoride induced testicular toxicities in adult Wistar rats

Cited by 10 publications

References 42 publications

Contribution of Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress and Autophagy Pathways to the Ameliorative Effects of Hesperidin in NaF‐Induced Testicular Toxicity

Contribution of Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress and Autophagy Pathways to the Ameliorative Effects of Hesperidin in NaF‐Induced Testicular Toxicity

Protective effect of platelet-rich plasma against structural and functional changes of the adult rat testis in carbimazole-induced hypothyroidism

Exposure to Fluoride From in Utero to Puberty Alters Gonadal Structure and Steroid Hormone Expression in Offspring Rats

Contact Info

Product

Resources

About