Fluoride induced leaky gut and bloom of Erysipelatoclostridium ramosum mediate the exacerbation of obesity in high-fat-diet fed mice

Chen, Guijie; Peng, Yujia; Huang, Yujie; Xie, Minhao; Dai, Zhuqing; Cai, Hao; Dong, Wei; Xu, Weiqi; Xie, Zhiyong; Chen, Dan; Fan, Xiaohui; Zhou, Wangting; Kan, Xuhui; Yang, Tingting; Chen, Chunxu; Sun, Yi; Zeng, Xiaoxiong; Z, Liu

doi:10.1016/j.jare.2022.10.010

Cited by 17 publications

(8 citation statements)

References 81 publications

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“…Studies have shown that Firmicutes and Bacteroides are the dominant bacteria in the gut ( Liu et al, 2021 ), and the increase in Firmicutes and decrease in Bacteroides may be a manifestation of obesity ( Ley et al, 2005 ; Magne et al, 2020 ), but feeding L-27-2, L-14-1, and F203 could slow this change, thus reducing the number of Firmicutes and increasing the number of Bacteroidetes in the gut of HFD mice. In addition, this experiment also found that feeding lactic acid bacteria could cause a decrease in Erysipelatoclostridium , which to some extent attenuated the increase in obesity in HFD mice ( Chen et al, 2023 ), and lactic acid bacteria also altered β-diversity in HFD mice, which is consistent with the results found by Yin et al (2018) . Taken together, these results suggest that the lactic acid bacteria strains isolated in the present experiment may achieve fat loss by altering the gut microbiota, thereby affecting intestinal cholesterol absorption, liver lipid metabolism, and the intestinal flora of mice, which was consistent with the former findings ( Wang et al, 2021 ).…”

Section: Discussionsupporting

confidence: 87%

Characterization and potential lipid-lowering effects of lactic acid bacteria isolated from cats

Liang,

Kang,

Zhao

et al. 2024

Front. Microbiol.

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IntroductionThis study aimed to study the characterization and the potential lipid-lowering effects of new isolated lactic acid bacteria from the feces of healthy adult cats.MethodsWe collected 85 cat fecal samples, isolated, screening lactic acid bacteria strains from samples, and investigated their in vitro and in vivo biological properties.ResultsA total of 221 lactic acid bacteria strains were isolated from 85 cat fecal samples. Sixteen strains with calcium dissolution rings greater than 1 mm were identified and selected for further characterization. Three lactic acid bacteria strains, Lactobacillus plantarum L-27-2, Pediococcus lactis L-14-1, and Enterococcus faecium, were identified as showing the most promising rates of cholesterol degradation (greater than 20%) and bacteriostatic radius (over 15 mm). These three strains exhibited robust growth and adherence to epithelial cells, along with adaptability to low pH (greater than 70%) and high bile salt conditions (greater than 60%), and remarkable cholesterol degradation and anti-pathogen activity. Sixteen mice were fed a high-fat diet (HFD) from 4 to 8 weeks of age, while a control group of the same size received a normal diet (ND). At 8 weeks of age, serum, feces and adipose tissue were collected. The results showed that, compared with mice fed an HFD diet alone, all mice fed an HFD diet plus lactic acid bacteria could decrease weight gain. P < 0.05 and the pathological changes of adipose tissue were alleviated. In addition, mice fed L-14-1 and F203 showed abdominal fat accumulation decreased (P < 0.05). Mice fed L-27-2 showed serum and liver triglyceride (TG) decreased (P < 0.05) and mice fed F203 showed serum high density lipoprotein cholesterol (HDL-C) increased (P < 0.01). mice fed L-27-2 and L-14-1 showed inflammatory cytokines (IL-6) was decreased (P < 0.01) Analysis of the fecal microbiota of mice fed these three lactic acid bacteria strains revealed alterations in the gut microbial community. There were common changes in intestinal microbes in mice fed these three lactic acid bacteria: (1) Bacteroides decreased; (2) Myxococcus increased; (3) Lachnoclostridium decreased. The microbes mentioned are all part of the core intestinal flora.DiscussionThis study provided three potential lactic acid bacteria for alleviating animal obesity and inflammation.

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Section: Discussionsupporting

confidence: 87%

Characterization and potential lipid-lowering effects of lactic acid bacteria isolated from cats

Liang,

Kang,

Zhao

et al. 2024

Front. Microbiol.

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“…36 Additionally, HFD may affect intestinal integrity and later the gut microbiotaproduced LPS can enter the circulation, thus inducing inflammation or metabolic disorders through the activation of nuclear factor kappa B (NF-κB). 37 Levels of LPS, TNF-⊍, IL-6 and IL-1⊎ apparently decreased within S. segobiensis DSM 27193-supplemented HFD-fed mice groups. Studies on LPS-induced cultured macrophages and HFD-fed mice found that POA had better anti-inflammatory capabilities than oleic acid, 38 which may be responsible for the inhibition of inflammatory cytokines by S. segobiensis DSM 27193.…”

Section: Discussionmentioning

confidence: 93%

“…This may due to several adipokines including leptin, resistin, and visfatin, which are synthesized in the adipose tissue; both enhance pro‐inflammatory factor generation and are related to the pathogenic mechanisms of obesity and its associated metabolic diseases 36 . Additionally, HFD may affect intestinal integrity and later the gut microbiota‐produced LPS can enter the circulation, thus inducing inflammation or metabolic disorders through the activation of nuclear factor kappa B (NF‐κB) 37 . Levels of LPS, TNF‐α, IL‐6 and IL‐1β apparently decreased within S. segobiensis DSM 27193‐supplemented HFD‐fed mice groups.…”

Section: Discussionmentioning

confidence: 99%

Palmitoleic acid‐rich oleaginous yeast Scheffersomyces segobiensis DSM 27193 exerts anti‐obesity effects by ameliorating hepatic steatosis and adipose tissue hypertrophy

Peng,

Zhang,

Bao

et al. 2023

J Sci Food Agric

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BACKGROUNDYeast biomass, encompassing fatty acids, terpenoids, vitamins, antioxidants, enzymes, and other bioactive compounds have been extensively utilized in food‐related fields. The safety and potential bioactivities of Scheffersomyces segobiensis DSM 27193, an oleaginous yeast strain, are unclear.RESULTSScheffersomyces segobiensis DSM 27193 accumulated large palmitoleic acid (POA) levels (43.4 g kg−1 biomass) according to the results of whole‐cell components. We annotated the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and predicted the categories and host of the pathogen–host interactions (PHI) genes in S. segobiensis DSM 27193. However, S. segobiensis DSM 27193 did not exert toxic effects in mice. Administration of S. segobiensis DSM 27193 led to substantial weight reduction by diminishing food intake in an obesity mouse model. Additionally, it reversed hepatic steatosis and adipose tissue hypertrophy, and improved abnormalities in serum biochemical profiles such as triglyceride, total cholesterol, low‐density lipoprotein cholesterol, lipopolysaccharide, tumor necrosis factor‐α, interleukin‐1β, and interleukin‐6.CONCLUSIONThis study is the first to illustrate the safety and effects of S. segobiensis DSM 27193 against obesity and offers a scientific rationale for its application in functional food supplements. © 2023 Society of Chemical Industry.

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“…This may suggest that fluoride’s potential metabolism-disrupting effects in children could be exacerbated by BMI. Furthermore, experimental research indicates a potential role of the gut microbiome in mediating fluoride-induced effects on obesity ( Chen et al, 2022 ). On the other hand, our study did not observe any associations with other anthropometric factors such as body fat, blood pressure, and waist circumference in pre-pubertal children.…”

Section: Discussionmentioning

confidence: 99%

Longitudinal associations between early-life fluoride exposures and cardiometabolic outcomes in school-aged children

India Aldana,

Colicino,

Cantoral Preciado

et al. 2024

Environment International

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Fluoride induced leaky gut and bloom of Erysipelatoclostridium ramosum mediate the exacerbation of obesity in high-fat-diet fed mice

Cited by 17 publications

References 81 publications

Characterization and potential lipid-lowering effects of lactic acid bacteria isolated from cats

Characterization and potential lipid-lowering effects of lactic acid bacteria isolated from cats

Palmitoleic acid‐rich oleaginous yeast Scheffersomyces segobiensis DSM 27193 exerts anti‐obesity effects by ameliorating hepatic steatosis and adipose tissue hypertrophy

Longitudinal associations between early-life fluoride exposures and cardiometabolic outcomes in school-aged children

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