Fluoride effects on bone formation and mineralization are influenced by genetics

Abstract

Introduction-A variation in bone response to fluoride (F − ) exposure has been attributed to genetic factors. Increasing fluoride doses (0ppm, 25ppm, 50ppm, 100ppm) for three inbred mouse strains with different susceptibilities to developing dental enamel fluorosis (A/J, a "susceptible" strain; SWR/J, an "intermediate" strain; 129P3/J, a "resistant" strain) had different effects on their cortical and trabecular bone mechanical properties. In this paper, the structural and material properties of the bone were evaluated to explain the previously observed changes in mechanical properties.

Autophagy may protect MC3T3-E1 cells from fluoride-induced apoptosis

“…Previous studies demonstrated that fluoride leads to the proliferation of osteoblasts, promotes their differentiation and modulates (20). Fluoride also increases alkaline phosphatase-specific activity and promotes bone formation in rats in vivo (21). However, excess fluoride may give rise to a number of adverse effects.…”

Differential Effects of Fluoride During Osteoblasts Mineralization in C57BL/6J and C3H/HeJ Inbred Strains of Mice

“…In B6 calvaria cells, there were signals more striking of fluoride effect than in the C3 osteoblasts [19], and the B6 calvaria cells were more responsive to inhibitory action of NaF. Some evaluations, in general between C3 and B6, have shown C3 mouse with more stability or resistance to factors/agents that can modify the bone metabolism [22,24,35,36].…”

“…Fluoride exposure showed no significant effect on bone microarchitecture in three mice strains [24]. Osteoid nodules formed in cultures of fetal rat calvarial cells exposed to fluoride inhibited the initiation phase during mineralization at low Fig.…”

Efeito do fluoreto no osso de camundongos com diferentes susceptibilidades genéticas à fluorose: uma análise proteômica

“…Os ossos imersos em NaF (0,145, 0,5 ou 2 M) apresentaram menor resistência e dureza, e uma maior deformação comparado ao osso não tratado, tanto na tensão (DEPAULA et al 2002;KOTHA et al 1998) quanto na compressão (WALSH;. Com altas doses de F (2,0 M NaF), um aumento significativo foi também observado na dureza (DEPAULA et al 2002) (CHACHRA et al 1999;MEYER 1978;HAILER 1998;MOUSNY et al 2008;TURNER et al 1997). …”

“…Para cada extrato foram realizados géis SDS, catiônico e aniônico, além do mapa proteômico. Esta linhagem foi escolhida por apresentar uma característica peculiar de resistência aos efeitos do F nos tecidos mineralizados (CARVALHO et al 2009;EVERETT et al 2002;MOUSNY et al 2006;MOUSNY et al 2008) ARAKAWA et al 1994;NEURATH;ERICKSON;COOPER 1942). O agente desnaturante funciona também como uma barreira para agregação durante o renovelamento da proteína, devido ao efeito caotrópico do desnaturante e as interações entre o desnaturante e a proteína.…”