Fluoride Dose Response in pH-Cycling Models Using Bovine Enamel

Vieira, Ana Elisa de Mello; Delbem, Alberto Carlos Botazzo; Sassaki, Kikue Takebayashi; Rodrigues, Eliana Aparecida; Cury, Jaime Aparecido; Cunha, Robson Frederico

doi:10.1159/000088189

Cited by 149 publications

(50 citation statements)

References 36 publications

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“…The cycles, in this case, are more frequent (6x 0.5 h in De/day and 6x 2.5 h in Re/day). Additionally, the addition of fluoride (0.03-0.06 ppm) into both de- and remineralizing solutions 3,11,75,86,103 has been proposed. For primary teeth, it is recommended to add higher fluoride concentrations in the de- and remineralizing solutions (0.25 ppm F) 94 .…”

Section: Results and Literature Reviewmentioning

confidence: 99%

pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations

et al. 2010

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Despite a plethora of in situ studies and clinical trials evaluating the efficacy of fluoridated dentifrices on caries control, in vitro pH cycling models are still broadly used because they mimic the dynamics of mineral loss and gain involved in caries formation. This paper critically reviews the current literature on existing pH-cycling models for the in vitro evaluation of the efficacy of fluoridated dentifrices for caries control, focusing on their strengths and limitations. A search was undertaken in the MEDLINE electronic journal database using the keywords "pH-cycling", "demineralization", "remineralization", "in vitro", "fluoride", "dentifrice". The primary outcome was the decrease of demineralization or the increase of remineralization as measured by different methods (e.g.: transverse microradiography) or tooth fluoride uptake. Inclusion of studies, data extraction and quality assessment were undertaken independently and in duplicate by two members of the review team. Disagreements were solved by discussion and consensus or by a third party. One hundred and sixteen studies were included, of which 42 addressed specifically the comparison of dentifrices using different pH-cycling models. The other studies included meta-analysis or reviews, data about the effect of different fluoride sources on de-remineralization, different methods for analysis de-remineralization and chemical variables and characteristics of dental hard tissues that might have influence on de-remineralization processes. Generally, the studies presented ability to detect known results established by clinical trials, to demonstrate dose-related responses in the fluoride content of the dentifrices, and to provide repeatability and reproducibility between tests. In order to accomplish these features satisfactorily, it is mandatory to take into account the type of substrate and baseline artificial lesion, as well as the adequate response variables and statistical approaches to be used. This critical review of literature showed that the currently available pH-cycling models are appropriate to detect dose-response and pH-response of fluoride dentifrices, and to evaluate the impact of new active principles on the effect of fluoridated dentifrices, as well as their association with other anti-caries treatments.

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Section: Results and Literature Reviewmentioning

confidence: 99%

pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations

et al. 2010

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“…2 H 2 O, 150 mmol/l KCl, 0.1 mol/l H 2 NC(CH 2 OH) 3 (TRIS), and 0.05 NaF. [21]Group 2 : Specimens were stored in 10°C cola in a refrigerator (Arcelik 4252N; Arcelik A.S. Istanbul, Turkey). A new bottle of cola was used in each period to maintain an acceptable level of carbonic gas.…”

Section: Methodsmentioning

confidence: 99%

Does the temperature of beverages affect the surface roughness, hardness, and color stability of a composite resin?

2013

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Objective:To investigate the effect of beverages’ temperature on the surface roughness, hardness, and color stability of a composite resin.Materials and Methods:Fifty specimens of the Filtek Z250 composite (3M ESPE, Dental Products, St.Paul, MN, USA) were prepared and initial roughness, microhardness, and color were measured. Then the specimens were randomly divided into five groups of 10 specimens each: Coffee at 70°C, coffee at 37°C, cola at 10°C, cola at 37°C, and artificial saliva (control). After the samples were subjected to 15 min × 3 cycles per day of exposure to the solutions for 30 days, the final measurements were recorded.Results:After immersion in beverages, the artificial saliva group showed hardness values higher than those of the other groups (P < 0.001) and the microhardness values were significantly different from the initial values in all groups except for the control group. Both cola groups showed roughness values higher than the baseline values (P < 0.05), while the other groups showed values similar to the baseline measurements. When ΔE measurements were examined, the 70°C coffee group showed the highest color change among all the groups (P < 0.05).Conclusion:High-temperature solutions caused alterations in certain properties of composites, such as increased color change, although they did not affect the hardness or roughness of the composite resin material tested.

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“…The artificial saliva used allows for rehardening of the slabs between the erosive challenges for simulation of the in vivo situation 31. However, under clinical conditions, human saliva is also responsible for the formation of the acquired pellicle, which is a physical barrier that protects the tooth against erosive attacks 32.…”

Section: Discussionmentioning

confidence: 99%

Effect of Acidic Challenge Preceded by Food Consumption on Enamel Erosion

et al. 2010

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Objectives:This in vitro study aimed to evaluate the effect of food consumption followed by acidic challenge on enamel erosion.Methods:Seventy-five enamel blocks obtained from bovine teeth were divided randomly into five groups (n=15 per group): GI – erosion with previous immersion in milk; GII – erosion with previous immersion in cheese extract; GIII – erosion with previous immersion in liver extract; GIV – erosion with previous immersion in broccoli extract; and GV – erosive effect of cola drink (control). Over 24 h, the slabs were submitted to 3 pH-cycles, each consisting of immersion in the studied food (GI to GIV) for 5 min followed by immersion in a cola drink for 5 min, and subsequently, the slabs were stored in artificial saliva (110 min). At the end of the pH-cycles, the slabs were stored in artificial saliva for 18 h. Enamel alterations were assessed by profilometry (μm). Data were tested using ANOVA and Scott-Knott’s tests (P<.05).Results:Mean erosion depths for enamel (μm) were 0.46 in GI, 0.55 in GII, 0.64 in GIII, 0.54 in GIV, and 1.18 in GVI. Enamel loss by acidic challenge alone (GV) was significantly higher than when the acidic challenges were preceded by food extract immersion.Conclusions:The data suggest that all studied foods could minimize the erosive effect on enamel.

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Fluoride Dose Response in pH-Cycling Models Using Bovine Enamel

Cited by 149 publications

References 36 publications

pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations

pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations

Does the temperature of beverages affect the surface roughness, hardness, and color stability of a composite resin?

Effect of Acidic Challenge Preceded by Food Consumption on Enamel Erosion

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