Fluoride-doped amorphous calcium phosphate nanoparticles as a promising biomimetic material for dental remineralization

Abstract

Demineralization of dental hard tissue is a widespread problem and the main responsible for dental caries and dentin hypersensitivity. The most promising strategies to induce the precipitation of new mineral phase are the application of materials releasing gradually Ca2+ and PO43− ions or mimicking the mineral phase of the host tissue. However, the design of formulations covering both processes is so far a challenge in preventive dentistry. In this work, we have synthesized innovative biomimetic amorphous calcium phosphate (ACP), which has been, for the first time, doped with fluoride ions (FACP) to obtain materials with enhanced anti-caries and remineralizing properties. Significantly, the doping with fluoride (F) did not vary the physico-chemical features of ACP but resulted in a faster conversion to the crystalline apatite phase in water, as observed by in-situ time-dependent Raman experiments. The efficacy of the as synthesized ACP and FACP samples to occlude dentinal tubules and induce enamel remineralization has been tested in vitro in human molar teeth. The samples showed good ability to partially occlude the tubules of acid-etched dentin and to restore demineralized enamel into its native structure. Results demonstrate that ACP and FACP are promising biomimetic materials in preventive dentistry to hinder demineralization of dental hard tissues.

Highly Porous Amorphous Calcium Phosphate for Drug Delivery and Bio-Medical Applications

“…In addition, ACP is bioactive, with better biodegradability than crystalline CaP and with the ability to promote osteoblast adhesion [10] and osteconductivity [11]. These properties make ACP a promising candidate material for bone regeneration [9,[12][13][14], in applications such as bone cements [15] and bio-ceramics [16,17].…”

“…Ion substitution (Mg 2+ , CO 3 2− , Sr 2+ , Zn 2+ , etc.) has also been widely used to stabilize synthetic ACP [13,14,20,21].…”

A Study of Fluoride-Containing Bioglass System for Dental Materials Derived from Clam Shell and Soda Lime Silica Glass

“…e high amounts of ion release will form apatite layer as the ionic activity increases during apatite nucleation process [52]. e formation of Ca and P ions is important in dental applications as it can increase the apatite saturation by enhancing the remineralization [53].…”

Apatite from NWA 10153 and NWA 10645—The Key to Deciphering Magmatic and Fluid Evolution History in Nakhlites

“…Raman spectroscopy is a suitable tool to observe the transformation of ACP to crystalline apatite for in situ experiments [59], stomatology [83], zoology [84,85], and medicine [86,87]. In all studies, this transformation is observed as a clear v 1 Raman band shift from~950 cm −1 (ACP marked band) to~960 cm −1 (apatite), and a sharpening of the 960 cm −1 band manifests as a decreasing full width at half maximum (FWHM) value, which is possibly caused by an increase in both crystallinity and lattice ordering during the transformation [59].…”