Fluorescently labeled mesenchymal stem cells (MSCs) maintain multilineage potential and can be detected following implantation into articular cartilage defects

Quintavalla, Joseph; Uziel-Fusi, Susan; Yin, Jian; Boehnlein, Ernst; Pastor, Gary; Blancuzzi, V.; Singh, H. N.; Kraus, Karl H.; O’Byrne, E. M.; Pellas, Theodore C.

doi:10.1016/s0142-9612(01)00086-2

Cited by 115 publications

(79 citation statements)

References 34 publications

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“…40 The chondrocytes were also detected in the defect up to 14 weeks after ACI using fluorescent dye. 41 Emans et al 42 demonstrated transplantation of chondrocyte or periosteal cell=scaffold constructs into an osteochondral defect and examined cell viability by fluorescent labeling 5 days after transplantation.…”

Section: Discussionmentioning

confidence: 99%

Variation of Mesenchymal Cells in Polylactic Acid Scaffold in an Osteochondral Repair Model

Oshima

Harwood²,

Coutts³

et al. 2009

Tissue Engineering Part C: Methods

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Objective: To achieve osteochondral regeneration utilizing transplantation of cartilage-lineage cells and adequate scaffolds, it is essential to characterize the behavior of transplanted cells in the repair process. The objectives of this study were to elucidate the survival of mesenchymal cells (MCs). In a polylactic acid (PLA) scaffold and assess the possibility of MC=PLA constructs for osteochondral repair. Design: Bone marrow from mature male rabbits was cultured for 2 weeks, and fibroblast-like MCs, which contain mesenchymal stem cells (MSCs), were obtained. A cell=scaffold construct was prepared with one million MCs and a biodegradable PLA core using a rotator device. One week after culturing, the construct was transplanted into an osteochondral defect in the medial femoral condyle of female rabbits and the healing process examined histologically. To examine the survivability of transplanted MCs, the male-derived sex-determining region Y (SRY) gene was assessed as a marker of MCs in the defect by polymerase chain reaction (PCR). Results: In the groups of defects without any treatment, and the transplantation of PLA without cells, the defects were not repaired with hyaline cartilage. The cartilaginous matrix by safranin O staining and type II collagen by immunohistochemical staining were recognized, however the PLA matrix was still present in the defects at 24 weeks after transplantation of the construct. During the time passage, transplanted MCs numbers decreased from 7.8Â105 at 1 week, to 3.5Â105 at 4 weeks, and to 3.8Â104 at 12 weeks. Transplanted MCs were not detectable at 24 weeks. Conclusions: MCs contribute to the osteochondral repair expressing the cartilaginous matrix, however the number of MCs were decreasing with time (i.e. 24 weeks). These results could be essential for achieving cartilage regeneration by cell transplantation strategies with growth factors and=or gene therapy.

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Section: Discussionmentioning

confidence: 99%

Variation of Mesenchymal Cells in Polylactic Acid Scaffold in an Osteochondral Repair Model

Oshima

Harwood²,

Coutts³

et al. 2009

Tissue Engineering Part C: Methods

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“…The later, identified as marrow stromal cells or mesenchymal stem cells (MSCs), not only support hematopoiesis but also differentiate along various mesodermal lineages to generate osteoblasts, chondrocytes, and adipocytes. [1][2][3] Because of these properties, MSCs have been exploited for their potential to repair or regenerate damaged tissues of mesenchymal origin, including bone fracture healing, 4 tendon repair, 5 cartilage regeneration, 6,7 and support of engraftment after chemotherapy. 8 One group has also identified a subpopulation of bone marrowderived cells, termed multipotent adult progenitor cells (MAPCs), which possess longer proliferative capacity and broader differentiation potential than MSCs, 9,10 including cells of all 3 germ layers in vitro, as well as the ability to generate chimeric mice when injected into blastocysts.…”

Section: Introductionmentioning

confidence: 99%

SSEA-4 identifies mesenchymal stem cells from bone marrow

Gang

Bosnakovski

Figueiredo

et al. 2006

Blood

469

350

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“…MSCs can be extracted from several organs, such as fetal liver, umbilical cord blood, bone marrow, placenta, adipose tissue, muscle, and dermis (Kraemer 1995), and can be induced to differentiate into cells of the articular cartilage Liechty et al 2000;Toma et al 2002) or chondrocyte lineages (Im et al 2001;Quintavalla et al 2002;Wakitani et al 1994). Furthermore, as IVD cells have phenotypes similar to those of chondrocytes, it would appear that IVD cells are differentiated from MSCs (Risbud et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Human mesenchymal stem cells implantation into the degenerated coccygeal disc of the rat

et al. 2009

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In this study, the authors explored the effect of human mesenchymal stem cell (MSC) implantation on the restoration of degenerative intervertebral discs (IVDs) in the rat. A unique rat coccygeal model was used to investigate the effects of transplanting human MSCs and to examine MSC survival in degenerative discs. MSC implantations into rat coccygeal IVDs were performed at 2 weeks postinjury. Radiologic and histologic evaluations were performed at 2, 4, 6, and 8 weeks post-injury. MSCinjected segments (TS) retained disc height and signal intensity, but injured non-injected segment (IS) progressively lost disc height. Pathological results revealed that the TS group showed relative restoration of the inner annulus structure; however, the IS group showed destruction of the inner annulus structure. Immunohistochemical staining using Anti-Human Nucleic Antibody (#MAB1281 Chemicon) revealed positive staining in the TS group at 2 weeks posttransplantation (4 weeks post-injury). This study shows that human MSCs survive for 2 weeks after transplantation into the IVDs of rats, and that MSCs increased the heights and signal intensities of intervertebral disc.

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Fluorescently labeled mesenchymal stem cells (MSCs) maintain multilineage potential and can be detected following implantation into articular cartilage defects

Cited by 115 publications

References 34 publications

Variation of Mesenchymal Cells in Polylactic Acid Scaffold in an Osteochondral Repair Model

Variation of Mesenchymal Cells in Polylactic Acid Scaffold in an Osteochondral Repair Model

SSEA-4 identifies mesenchymal stem cells from bone marrow

Human mesenchymal stem cells implantation into the degenerated coccygeal disc of the rat

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