Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture

Krahn, K Katy Nash; Bouten, Cvc Carlijn; Tuijl, S Sjoerd van; Zandvoort, M Marc van; Merkx, Maarten

doi:10.1016/j.ab.2006.01.013

Cited by 152 publications

(150 citation statements)

References 46 publications

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“…The collagen distribution within the constructs was evaluated with a CNA staining technique (Krahn et al 2006) using a Confocal Laser Scanning Microscope (Carl Zeiss, Oberkochen, Germany). Briefly, samples were labeled with Cell Tracker Orange (CTO; Invitrogen Molecular Probes) and CNA35-OG488 (CNA), to fluorescently stain cell cytoplasm and collagen, respectively.…”

Section: Engineered Constructsmentioning

confidence: 99%

Modeling the impact of scaffold architecture and mechanical loading on collagen turnover in engineered cardiovascular tissues

Argento

Jonge

Söntjens

et al. 2014

Biomech Model Mechanobiol

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Section: Engineered Constructsmentioning

confidence: 99%

Modeling the impact of scaffold architecture and mechanical loading on collagen turnover in engineered cardiovascular tissues

Argento

Jonge

Söntjens

et al. 2014

Biomech Model Mechanobiol

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“…Subsequently, these four annulus samples were incubated at 37 • C overnight in PBS containing a final concentration of 8 µM CNA35OG488. The recently developed CNA35-OG488 probe binds to a wide range of collagen (type I to VI) with different fluorescent intensities and has little cross-reactivity with non-collagenous extra-cellular matrix proteins (Krahn et al 2006). The CNA35 probe is Oregon Green-labelled and is used to visualize the extra-cellular matrix.…”

Section: Specimen Preparationmentioning

confidence: 99%

3D non-affine finite strains measured in isolated bovine annulus fibrosus tissue samples

Huyghe

Jongeneelen

2011

Biomech Model Mechanobiol

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Understanding of the mechanics of disc tissue calls for measurement of strains in physiological conditions. Because the intervertebral disc is gripped between two vertebrae, the swelling is constrained in vivo, resulting in a intradiscal pressure of 0.1-0.2 MPa in supine position. The excision of isolated disc tissue samples results often in nonphysiological swelling. The purpose of the present study is to measure 3D finite strains in isolated bovine disc tissue specimens under physiological osmolarity and pressure, particularly around discontinuities of the collagen network. The collagen is stained by means of CNA35 probe, and the (dead) cells are stained by means of propidium iodide. The tissue is observed under confocal microscopy, under an externally applied pressure generated by a PEG solution. The 3D finite strains are obtained through correlation of the texture of the 3D images. The correlation technique yields principal strains in all areas except within collagen-free areas. The deformation is strongly non-affine. Especially around discontinuities, the strain field is non-homogeneous. Macroscopic strains as computed from finite element analysis of whole discs are insufficient to predict microstrains around clefts or cells. Because of the small number of specimens, the present results should be considered preliminary.

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“…Finally, microscopic inspection requires some sort of staining, presumably with fluorescent dyes in several mm thick constructs. Some fluorescent dyes, such as the CNA35 for collagen [98], can be used during culture. However, the use of such probes is limited to in vitro cultures and cannot be used in implants.…”

Section: Current and Future Developmentsmentioning

confidence: 99%

Review on Patents for Mechanical Stimulation of Articular Cartilage Tissue Engineering

Donkelaar¹,

Schulz

2008

BIOMENG

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Abstract:To repair articular cartilage defects in osteoarthritic patients with three-dimensional tissue engineered chondrocyte grafts, requires the formation of new cartilage with sufficient mechanical properties. The premise is that mechanical stimulation during the culturing process is necessary to reach this aim. Therefore, mechanical stimulation systems have been integrated in aseptic bioreactors for in vitro cultivation of tissue engineered cartilage. These vary from simple unconfined compression systems to advanced bioreactors in which deformation and loading are fully controlled. Fluid handling in these devices is another decisive parameter for the success of cartilage tissue engineering.Over the last decades bioreactor developments have resulted in the filing of many patents. The aim of this paper is to review these patents, categorize them according to their possibilities for mechanical stimulation and fluid handling systems and finally to discuss them in the context of the demands of a functional tissue engineered cartilage from a mechanical perspective.

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Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture

Cited by 152 publications

References 46 publications

Modeling the impact of scaffold architecture and mechanical loading on collagen turnover in engineered cardiovascular tissues

Modeling the impact of scaffold architecture and mechanical loading on collagen turnover in engineered cardiovascular tissues

3D non-affine finite strains measured in isolated bovine annulus fibrosus tissue samples

Review on Patents for Mechanical Stimulation of Articular Cartilage Tissue Engineering

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