Fluorescent Turn-on Aptasensor of Staphylococcus aureus Based on the FRET Between Green Carbon Quantum Dot and Gold Nanoparticle

Pebdeni, Azam Bagheri; Hosseini, Morteza; Ganjali, Mohammad Reza

doi:10.1007/s12161-020-01821-4

Cited by 63 publications

(16 citation statements)

References 42 publications

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“…However, the use of traditional organic fluorescent dyes in sensors is limited due to low fluorescence intensity and rapid photobleaching. Compared with traditional organic fluorescent dyes, fluorescent nanomaterials such as quantum dots (QDs) ( Fu et al, 2020 ; Tao et al, 2021 ), upconversion nanoparticles (UCNPs) ( Wu et al, 2014 ; Ouyang et al, 2021b ) and carbon dots ( Cui et al, 2019 ; Pebdeni et al, 2020 ) that have emerged in recent years have many advantages, such as wider absorption spectra, size-tunable narrower emission spectra and better stability ( Yao et al, 2014 ).…”

Section: Aptasensors Based On Optical Transductionmentioning

confidence: 99%

Recent Advances in Aptasensors For Rapid and Sensitive Detection of Staphylococcus Aureus

Chen

Lai

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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The infection of Staphylococcus aureus (S.aureus) and the spread of drug-resistant bacteria pose a serious threat to global public health. Therefore, timely, rapid and accurate detection of S. aureus is of great significance for food safety, environmental monitoring, clinical diagnosis and treatment, and prevention of drug-resistant bacteria dissemination. Traditional S. aureus detection methods such as culture identification, ELISA, PCR, MALDI-TOF-MS and sequencing, etc., have good sensitivity and specificity, but they are complex to operate, requiring professionals and expensive and complex machines. Therefore, it is still challenging to develop a fast, simple, low-cost, specific and sensitive S. aureus detection method. Recent studies have demonstrated that fast, specific, low-cost, low sample volume, automated, and portable aptasensors have been widely used for S. aureus detection and have been proposed as the most attractive alternatives to their traditional detection methods. In this review, recent advances of aptasensors based on different transducer (optical and electrochemical) for S. aureus detection have been discussed in details. Furthermore, the applications of aptasensors in point-of-care testing (POCT) have also been discussed. More and more aptasensors are combined with nanomaterials as efficient transducers and amplifiers, which appears to be the development trend in aptasensors. Finally, some significant challenges for the development and application of aptasensors are outlined.

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Section: Aptasensors Based On Optical Transductionmentioning

confidence: 99%

Recent Advances in Aptasensors For Rapid and Sensitive Detection of Staphylococcus Aureus

Chen

Lai

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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“…The method was successfully used to detect S. aureus in milk and orange juice with a linear range of 10–10 8 CFU mL −1 and detection limit as low as 10 CFU mL −1 . [ 134 ]…”

Section: The Application Of Green Carbon Dots In Analysis and Detectionmentioning

confidence: 99%

Application Progress of Green Carbon Dots in Analysis and Detection

Zuo

et al. 2022

Part & Part Syst Charact

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Green chemistry is currently attracting more and more attention due to its eco‐friendly, and green carbon dots (g‐CDs) are creating a research boom. The g‐CDs have huge application prospects in analysis and detection due to their excellent optical properties, high biocompatibility, and low toxicity compared to other fluorescent materials. Based on the study of g‐CDs, the optical properties, synthesis methods, surface modification methods, and sensing mechanisms of g‐CDs are outlined, and the application in analysis and detection of environmental, food, biomolecules, and other organic small molecules are reviewed. At the same time, the challenges faced by g‐CDs are outlined. This review provides valuable references for further research and wider application of g‐CDs.

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“…Aptamers are functional single-stranded nucleic acids obtained through multiple rounds of screening [ 28 , 29 ]. The binding specificity of aptamer and target is high and stable, and each target can bind thousands of aptamers on its surface [ 30 ]. However, aptamers may intertwine with each other, affecting their recognition of the target [ 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Sandwich Fluorescence Detection of Foodborne Pathogen Staphylococcus aureus with CD Fluorescence Signal Amplification in Food Samples

Ping

et al. 2022

Foods

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Timely detection of Staphylococcus aureus (S. aureus) is critical because it can multiply to disease−causing levels in a matter of hours. Herein, a simple and sensitive DNA tetrahedral (Td) fluorescence signal amplifier with blue carbon quantum dots (bCDs) was prepared for sandwich detection of S. aureus. bCD was modified at the apex of Td, and an aptamer on Td was used to accurately identify and “adsorb” the amplifier to the surface of S. aureus. Atomic force microscopy (AFM) demonstrates the successful preparation of this signal amplifier. The fluorescence intensity emitted in this strategy increased 4.72 times. The strategy showed a stronger fluorescence intensity change, sensitivity (linear range of 7.22 × 100–1.44 × 109 CFU/mL with a LOD of 4 CFU/mL), and selectivity. The recovery rate in qualified pasteurized milk and drinking water samples was 96.54% to 104.72%. Compared with simple aptamer sandwich detection, these fluorescence signal amplifiers have improved fluorescence detection of S. aureus. Additionally, this fluorescent signal amplification strategy may be applied to the detection of other food pathogens or environmental microorganisms in the future.

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Fluorescent Turn-on Aptasensor of Staphylococcus aureus Based on the FRET Between Green Carbon Quantum Dot and Gold Nanoparticle

Cited by 63 publications

References 42 publications

Recent Advances in Aptasensors For Rapid and Sensitive Detection of Staphylococcus Aureus

Recent Advances in Aptasensors For Rapid and Sensitive Detection of Staphylococcus Aureus

Application Progress of Green Carbon Dots in Analysis and Detection

Sandwich Fluorescence Detection of Foodborne Pathogen Staphylococcus aureus with CD Fluorescence Signal Amplification in Food Samples

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