Fluorescent Staining of the Male Chromatin Body in Interphase Human Nuclei

Abstract

SummaryA fluorescence staining method of the male chromatin body in human interphase nuclei is described.

Evidentiation of the Male Chromatin Body in Human Peripheral Blood Leukocytes — A Quick Method for the Identification of Genetic Sex

“…Thanks to this elective affinity for some acridine derivatives, it has been possible to identify the Y-chromosome in interphase human nuclei. In fact, an intensely fluorescent body (F-body), corresponding to the distal part of the long arm of the Y-chromosome, is present in the nuclei of the oral mucosa of normal males and in cultured fibroblasts and lymphocytes (Pearson et al 1970, Moscetti and Mastroianni 1971, Majewski et al 1971). It has further been possible to evidence the F-body in human spermatozoa (Barlow and Vosa 1970) and in cells deriving from the amniotic fluid of male foetuses (Caspersson et al 1970).…”