Fluorescent sensing of nucleus density assists in identifying tumor cells using an AIE luminogen

Situ, Bo; He, Bo; Xu, Chen; He, Xiaojing; Feng, Weiwei; Ye, Xinyi; Huang, Yue; Xue, Xiaolei; Liu, Shan; Xia, Qiong; Zou, Jianjun; Tang, Ben Zhong; Zheng, Lei

doi:10.1016/j.cej.2020.128183

Cited by 9 publications

(4 citation statements)

References 48 publications

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“…Although there is an ongoing debate regarding the microviscosity of the nucleoplasm and cytoplasm, mostly related to the size of the used probe and the crowding and the heterogeneity of the environment, several studies have indicated that their viscosities differ, the former being more viscous than the latter. ,,− A fluorescence correlation spectroscopy study has reported on a nucleoplasm viscosity for HeLa and lung adenocarcinoma (ASTC-a-1) cell lines that were 3 to 4 times larger in comparison to water at 37 °C . The authors also reported ∼8% lower values for the viscosity of the cytoplasm in comparison to the nucleoplasm.…”

Section: Resultsmentioning

confidence: 99%

Formation of Highly Emissive Anthracene Excimers for Aggregation-Induced Emission/Self-Assembly Directed (Bio)imaging

Pacheco-Liñán,

Alonso-Moreno,

Ocaña

et al. 2023

ACS Appl. Mater. Interfaces

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AIEgens have emerged as a promising alternative to molecular rotors in bioimaging applications. However, transferring the concept of aggregation-induced emission (AIE) from solution to living systems remains a challenge. Given the highly heterogeneous nature and the compartmentalization of the cell, different approaches are needed to control the self-assembly within the crowded intricate cellular environment. Herein, we report for the first time the selfassembly mechanism of an anthracene-guanidine derivative (AG) forming the rare and highly emissive T-shaped dimer in breast cancer cell lines as a proof of concept. This process is highly sensitive to the local environment in terms of polarity, viscosity, and/or water quantity that should enable the use of the AG as a fluorescence lifetime imaging biosensor for intracellular imaging of cellular structures and the monitoring of intracellular state parameters. Different populations of the monomer and T-shaped and π−π dimers were observed in the cell membrane, cytoplasm, and nucleoplasm, related to the local viscosity and presence of water. The T-shaped dimer is formed preferentially in the nucleus because of the higher density and viscosity compared to the cytoplasm. The present results should serve as a precursor for the development of new design strategies for molecular systems for a wide range of applications such as cell viscosity, density, or temperature sensing and imaging.

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Section: Resultsmentioning

confidence: 99%

Formation of Highly Emissive Anthracene Excimers for Aggregation-Induced Emission/Self-Assembly Directed (Bio)imaging

Pacheco-Liñán,

Alonso-Moreno,

Ocaña

et al. 2023

ACS Appl. Mater. Interfaces

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“…[34] After studying this anomaly phenomenon, Tang et al innovatively advanced the concept of aggregation-induced emission (AIE), and many pioneering contributions in fields of AIE luminescence mechanisms and bioapplications have been made by his group. [35][36][37][38] In addition, a large number of studies have shown that AIE fluorogens exhibit higher photostability, extremely high signal reliability, and lower background signal compared with traditional fluorophores. As we have seen, tremendous activatable AIE probes designed on the principle of chemical reactivity for detecting analytes of interest and sensing biological analytes have been reported in the past decade.…”

Section: Introductionmentioning

confidence: 99%

Activity‐based smart AIEgens for detection, bioimaging, and therapeutics: Recent progress and outlook

et al. 2021

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Activity‐based approaches for designing AIEgens possess prominent advantages including high selectivity, sensitivity, and signal‐to‐noise ratio, and they have received more attention in recent years. Excellent activatable AIE probes have been reported for detecting toxic substances, imaging intracellular active molecules/biomolecules, as well as monitoring the activity of overexpressed enzymes in cancers. Moreover, the majority of activatable theranostic AIEgens can be specifically triggered in cancer cells and can kill these cells under light irradiation, while they have no distinct effect on normal cells, demonstrating satisfactory therapeutic selectivity that is superior to that of traditional chemotherapy. Thus, in this review, we systematically summarized the development of activatable AIE bioprobes in recent years from molecular design principles to biological applications. The challenges of activatable AIE probes and the corresponding solutions are described. We hope that the information provided in this review will facilitate the design of more activatable AIE probes to promote practical application of corresponding AIEgens.

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“…26 Situ et al prepared a small positively charged molecular probe, MASPB, with AIE features for lighting up the nucleolus of cancer cells. 27 Cheng et al reported NLS peptide-decorated AIEgens for long-term nucleus imaging in cancer cells. 28 However, the design and development of near-infrared (NIR) AIE-active fluorescent probes with low cost, good water solubility, a compact structure, and a small size for nucleus imaging in living cells are still challenging tasks.…”

mentioning

confidence: 99%

Malachite green: a long-buried water-soluble AIEgen with near-infrared fluorescence for living cell nucleus staining

Luo,

Zhou,

et al. 2024

Chem. Commun.

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Fluorescent sensing of nucleus density assists in identifying tumor cells using an AIE luminogen

Cited by 9 publications

References 48 publications

Formation of Highly Emissive Anthracene Excimers for Aggregation-Induced Emission/Self-Assembly Directed (Bio)imaging

Formation of Highly Emissive Anthracene Excimers for Aggregation-Induced Emission/Self-Assembly Directed (Bio)imaging

Activity‐based smart AIEgens for detection, bioimaging, and therapeutics: Recent progress and outlook

Malachite green: a long-buried water-soluble AIEgen with near-infrared fluorescence for living cell nucleus staining

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