Fluorescent properties of nanodiamonds in result of interactions of nanodiamonds with biomacromolecules in water

Vervald, Ekaterina N.; Vervald, Alexey M.; Laptinskiy, Kirill; Dolenko, Tatiana A.

doi:10.1117/12.2306592

Cited by 2 publications

(3 citation statements)

References 28 publications

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“…Earlier, we showed that protic solvents quench the DND fluorescence. , Moreover, the stronger the hydrogen bonds and the greater their number in the solvent, the greater the quenching of the fluorescence of the nanoparticles. In accordance with these results, the rise of DND fluorescence during the adsorption of lysozymes on its surface can be explained by the screening of this surface from water, i.e., reduction of quenching of DND fluorescence by water. , This is directly evidenced by the course of the dependences of the DND fluorescence parameter F 0 on the concentration of lysozymes in suspension (Figure b). As the amount of lysozyme adsorbed on the surface of nanoparticles (from 0 to a lysozyme concentration in the suspension of 0.10–0.15 g/L) increases, the interactions of the surface groups of DNDs with surrounding water molecules cease to be, and as a result, a sharp increase in DND fluorescence is observed.…”

Section: Resultssupporting

confidence: 78%

“…Moreover, for larger nanoparticles, increase in the fluorescence intensities of the complexes was stronger. In our previous work, it was found that a small amount of adsorbed lysozyme can also decrease the fluorescence of NDs of sizes of 5 and 10 nm, while further adsorption of lysozyme, however, leads to an established increase in the fluorescence of nanoparticles.…”

Section: Introductionmentioning

confidence: 96%

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Bilayer Adsorption of Lysozyme on Nanodiamonds in Aqueous Suspensions

Vervald

Burikov

et al. 2020

J. Phys. Chem. C

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The interactions of one of the most famous enzymes, lysozyme, with carboxylated nanodiamonds in water were studied. It was found that stable complexes are formed as a result of lysozyme adsorption on the surface of nanodiamonds. Based on the obtained adsorption isotherms and change in the fluorescence of nanodiamonds during the adsorption of lysozyme on them, it is concluded that lysozyme is adsorbed on carboxylated nanodiamonds in two layers. Numerical estimates and IR absorption spectroscopy data showed that the lysozyme has different adsorption orientations in the first and second layers, with preferential side-on and end-on orientations, correspondingly. Moreover, in the first layer, lysozyme undergoes significant conformational changes. The enzymatic activity of adsorbed lysozyme in both layers is discussed.

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Section: Resultssupporting

confidence: 78%

Section: Introductionmentioning

confidence: 96%

Bilayer Adsorption of Lysozyme on Nanodiamonds in Aqueous Suspensions

Vervald

Burikov

et al. 2020

J. Phys. Chem. C

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“…Photoluminescence (PL) of NDs and CDs is also dependent on the properties of nanoparticles’ surfaces and their immediate environment. For NDs, the depednece of PL on their surface chemistry, − on the strength of the environments’ hydrogen bonds, ,, and on interactions with biomolecules ,− allowed us to distinguish the specific type of ND emission:surface photoluminescence, distinct from the well-studied PL of color centers in the diamond core. − Recently, increasingly more authors believe that the source of surface PL of nanodiamonds is the functionalized sp 2 -hybridized carbon on their surfaces. − …”

Section: Introductionmentioning

confidence: 99%

Surface Photoluminescence of Oxidized Nanodiamonds: Influence of Environment pH

et al. 2021

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Nanoparticles’ environment, in particular pH, can strongly affect their photoluminescence (PL). This can be especially true for the surface PL of nanodiamonds (NDs) known to be dependent on the composition of surface groups. In this paper, the effect of environmental pH in the range from 2 to 12.5 on the surface photoluminescence of oxidized NDs of various syntheses and surface treatments was investigated. For the first time, the varying changes of the NDs’ PL with the pH were shown, having, however, an essential common feature: they were observed in the same pH regions from 2 to 5 and from 9 to 12.5, while in the pH region from 5 to 9, the PL intensity of all samples was almost constant. Obtained dependences of NDs’ ζ-potentials on pH and quantum mechanical modeling showed that all changes of NDs’ PL are due to the (de)protonation of surface groups: carboxyl at pH from 2 to 5 and hydroxyl on sp2-hybridized carbon at pH from 9 to 12.5. The varying response of NDs’ PL to such deprotonation shows the possibility of tuning the surface photoluminescence of NDs for use in industry and biomedicine beyond what is possible by only controlling the nanodiamonds’ surface groups.

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Fluorescent properties of nanodiamonds in result of interactions of nanodiamonds with biomacromolecules in water

Cited by 2 publications

References 28 publications

Bilayer Adsorption of Lysozyme on Nanodiamonds in Aqueous Suspensions

Bilayer Adsorption of Lysozyme on Nanodiamonds in Aqueous Suspensions

Surface Photoluminescence of Oxidized Nanodiamonds: Influence of Environment pH

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