Fluorescent probes sensitive to changes in the cholesterol-to-phospholipids molar ratio in human platelet membranes during atherosclerosis

Abstract

Environment-sensitive fluorescent probes were used for the spectroscopic visualization of pathological changes in human platelet membranes during cerebral atherosclerosis. It has been estimated that the ratiometric probes 2-(2'-hydroxyphenyl)-5-phenyl-1,3,4-oxadiazole and 2-phenyl-phenanthr[9,10]oxazole can detect changes in the cholesterol-to-phospholipids molar ratio in human platelet membranes during the disease.

Fluorescent probes for imaging: a focus on atherosclerosis

“…To surmount this problem, the development of sensors with two or more analytes can provide more atherosclerosis pathological information and improve the diagnostic accuracy. 50,51 Ye et al 52 were the first to propose the dual-target sequentially activated fluorescent probe (MTB-B-CF 3 ) that enables highly specific and sensitive detection of LDs and HClO via "off-on" and ratiometric readouts, respectively (Fig. 2A).…”

Two-Color Visualization of Cholesterol Fluctuation in Plasma Membranes by Spatial Distribution-Controllable Single Fluorescent Probes

“…As one of the important components of the plasma membrane, cholesterol (CL) plays an indispensible role in live cells. , Particularly, most of the CL is present in plasma membranes and it is associated with important biological processes in plasma membranes, including endocytosis, exocytosis, and signaling. , Furthermore, CL in plasma membranes interacts with membrane lipids, tunes phase transition points of lipids systems, and maintains the fluidity and strength of plasma membranes concurrently. , Minor changes in the CL levels can bring significant influences on the membrane permeability, fluidity, strength, and many other physiological properties. , On the other hand, many diseases were closely linked with the abnormal levels of CL. Decreased levels of CL contents are closely related with depression, cancer, and cerebral hemorrhage. , Elevated concentration of CL can induce atherosclerosis and increase the risk of heart attack, stroke, and peripheral vascular disease. , Therefore, the selective detection of CL levels in plasma membranes is a significant task for in-depth investigations in pathology and biology.…”

“…9,10 Elevated concentration of CL can induce atherosclerosis and increase the risk of heart attack, stroke, and peripheral vascular disease. 11,12 Therefore, the selective detection of CL levels in plasma membranes is a significant task for in-depth investigations in pathology and biology.…”

Fluorescence research in Ukraine

“…Posokhov has used the environment-sensitive oxazole and oxadiazole based, ratiometric fluorescent probes to estimate pathological changes in human platelet membranes during cerebral atherosclerosis [28]. These probes enable quantification of the cholesterolto-phospholipids molar ratio in human platelet membranes during the disease.…”