Fluorescent probe for Fe(III) with high selectivity and its application in living cells

Recent Advances on Iron(III) Selective Fluorescent Probes with Possible Applications in Bioimaging

“…The concentration of Fe 3+ is determined in various real water samples and is successfully applied to monitor intracellular Fe 3+ ion in living cells. The probe 28 is designed by reaction rhodamine hydrazide with two equivalents of 2-(thiophen-2-yl)acetyl chloride [36]. The turn-on fluorescence from 28 can be applied to detect Fe 3+ down to 0.13 μM, and the probe is suitable in the pH range from 4 to 9.…”

Fluorescence Sensors for Fe3+ Ion with High Selectivity and Sensitivity and Bioimaging in Living Cells

“…In order to study the binding stoichiometry between L1 and Fe 3+ ion, the Job's plot analysis experiment based on fluorescence intensity was carried out by keeping the total concentration of L1 with Fe 3+ at 1.00×10 −4 M, and consequent changing the molar ration of Fe 3+ ion ([Fe 3+ ]/([L1] + [Fe 3+ ])=0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0). The results revealed that when the mole fraction of Fe 3+ was 0.5, the fluorescence intensity of L1 reached the maximum value (Figure b), indicating that the ratio at which L1 complex with Fe 3+ was 1:1 ,…”

“…In comparison with previously reported fluorescence sensors for Fe 3+ detection, both L1 and L2 displayed superior sensitivities in terms of detection limit, high selectivity and applicability in live cell imaging. As shown in Table , each sensor had certain advantages, such as rare interference,,,,,, low detection limits,,, single‐step synthesis, and applicability in live cell imaging ,,,,,. Another advantage of these sensors was that they could be used in aqueous media without interference from other ions, which led us to achieve the fluorescence detection of Fe 3+ in living cells at micromolar level.…”

Rhodamine‐Based Fluorescence ‘Turn‐On’ Chemosensor: Detection of Fe3+Ion in Aqueous Medium and MCF‐7 Live Cells

“…Recently, fluorescent probes have gained much attention and are being utilized as efficient sensors in environment and cell biology for their fast, easy and real time detection mechanism [48,49] . There are many efficient probes reported for detection of Fe 3+ , however very few can work in aqueous medium [50–57] . Rhodamine derivatives with their significant photophysical properties, high quantum yield, water solubility and colour development properties have been efficiently employed as fluorescent sensors [53,54] .…”