Fluorescent paper strip immunoassay with carbon nanodots@silica for determination of human serum amyloid A1

Supianto, Mulya; Lee, Sang Hyuk; Jhung, Sung Hwa; Mohammad, Hazara Begum; Vu, Hung M.; Kim, Min‐Sik; Song, Woo‐Young; Kim, Tae‐Young; Lee, Hye Jin

doi:10.1007/s00604-021-05019-1

Cited by 9 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fluorescence lateral flow immunoassay (FLFIA) is a type of immunoassay based on fluorescent nanoparticles (carbon dots, inorganic quantum dots, and fluorescent polystyrene microsphere) and exhibits simplicity, quantitation, and sensitivity . Most conventional fluorescent dyes suffer from the inherent phenomenon of aggregation-caused quenching (ACQ) owing to π–π stacking and the formation of intermolecular dimers, emerging low fluorescence signal in the aggregated state .…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive and Ultrastable Lateral Flow Immunoassay Based on Polydopamine-Coated Aggregation-Induced Emission Fluorescent Microspheres with Excellent Fluorescence Performance and Biofriendly Coupling Strategy

Zhang

Lai

et al. 2023

Chem. Mater.

View full text Add to dashboard Cite

Conventional fluorescent microspheres (CFMs) have the disadvantages of low photoluminescence intensity (aggregation-caused quenching) and poor antibody conjugation. Herein, we achieved the improved performance of lateral flow immunoassay (LFIA) based on the high-fluorescent property of aggregation-induced emission fluorescent microspheres (AIEFMs) and biofriendly antibody coupling strategy of the polydopamine (PDA) layer. Although the PDA layer quenches the fluorescence intensity of AIEFM by Förster resonance energy transfer (FRET), quenching can be effectively controlled by ingenious adjusting of the thickness of the PDA. The PDA-coated AIEFM (AIEFM@PDA), which not only retained the strong fluorescence of AIEFM but also improved the antibody coupling efficiency and reproducibility of LFIAs, was successfully applied in sandwich and competitive LFIAs for the highly sensitive detection of pathogenic bacteria (Escherichia coli O157:H7) and antibiotics (enrofloxacin). In comparison with the CFM method, the proposed AIEFM@PDA-LFIA for the detection of E. coli O157:H7 and enrofloxacin could enhance the sensitivity by 40 times and 20 times, respectively. In addition, AIEFM@PDA-LFIA was further used for the detection of E. coli O157:H7 in river water, apple juice, and milk with satisfactory recoveries from 82.24 to 123.02% and enrofloxacin in pork, chicken, fish, and beef with satisfactory recoveries from 75.67 to 120.89%. The proposed AIEFM@PDA-LFIA showed excellent potential in rapid detection applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Highly Sensitive and Ultrastable Lateral Flow Immunoassay Based on Polydopamine-Coated Aggregation-Induced Emission Fluorescent Microspheres with Excellent Fluorescence Performance and Biofriendly Coupling Strategy

Zhang

Lai

et al. 2023

Chem. Mater.

View full text Add to dashboard Cite

show abstract

“…Because immunoassays are often run directly in blood or saliva, protein coronas are definitely present in immunoassays. There is evidence in the literature of the protein corona impacting immunoassays that use fluorescence readouts . Characterization of protein coronas by SDS-PAGE and LC–MS/MS has shown that only 20–30% adsorbed proteins on a NP surface are able to bind to a target …”

Section: Interface Issues For Biosensorsmentioning

confidence: 99%

“…There is evidence in the literature of the protein corona impacting immunoassays that use fluorescence readouts. 85 Characterization of protein coronas by SDS-PAGE and LC−MS/MS has shown that only 20−30% adsorbed proteins on a NP surface are able to bind to a target. 86 Some approaches have utilized surface passivation chemistries to mitigate the protein corona and nonspecific adsorption to NPs, which is usually performed after conjugation as a backfill step.…”

Section: ■ Interface Issues For Biosensorsmentioning

confidence: 99%

Nanomaterial and Interface Advances in Immunoassay Biosensors

Calidonio

Gómez-Márquez²,

Hamad‐Schifferli

2022

J. Phys. Chem. C

View full text Add to dashboard Cite

Biosensors have been used for a remarkable array of applications, including infectious diseases, environmental monitoring, cancer diagnosis, food safety, and numerous others. In particular, the global COVID-19 pandemic has exposed a need for rapid tests, so the type of biosensor that has gained considerable interest recently is immunoassays, which are used for rapid diagnostics. The performance of paper-based lateral flow and dipstick immunoassays is influenced by the physical properties of the nanoparticles (NPs), NP−antibody conjugates, and paper substrate. Many materials innovations have enhanced diagnostics by increasing the sensitivity or enabling unique readouts. However, negative side effects can arise at the interface between the biological sample and biomolecules and the NP or paper substrate, such as nonspecific adsorption and protein denaturation. In this Perspective, we discuss the immunoassay components and highlight chemistry and materials innovations that can improve sensitivity. We also explore the range of biointerface issues that can present challenges for immunoassays.

show abstract

“…In order to meet the requirements of a more accurate and sensitive analysis, the combination of fluorescent microspheres as labelling probes and portable strip readers has become a research hotspot in recent years. Quantum dots (QDs), carbon nanodots-silica [ 33 ], upconverting nanoparticles [ 34 ], fluorescein isothiocyanate (FITC) [ 35 ] and lanthanide-labeled fluorescent nanoparticles [ 36 ], as substitutes for colloidal gold, have been widely used in applications in clinical detection [ 37 ], molecular biology [ 38 ] and other related fields. Europium nanoparticle (EuNP) luminous material has been widely employed because of its benefits of ultra-long emission half-life, massive Stokes shift, longer measurement duration, removal of background fluorescence and better detection sensitivity [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

Europium Nanoparticles-Based Fluorescence Immunochromatographic Detection of Three Abused Drugs in Hair

Li³

et al. 2023

Toxics

View full text Add to dashboard Cite

Drug abuse is becoming increasingly dangerous nowadays. Morphine (MOP), methamphetamine (MET) and ketamine (KET) are the most commonly abused drugs. The abuse of these drugs without supervision can cause serious harm to the human body and also endanger public safety. Developing a rapid and accurate method to screen drug suspects and thus control these drugs is essential to public safety. This paper presents a method for the simultaneous quantitative detection of these three drugs in hair by a europium nanoparticles-based fluorescence immunochromatographic assay (EuNPs−FIA). In our study, the test area of the nitrocellulose membrane was composed of three equally spaced detection lines and a quality control line. The test strip realized the quantitative analysis of the samples by detecting the fluorescence brightness of the europium nanoparticles captured on the test line within 15 min. For the triple test strip, the limits of detection of MOP, KET and MET were 0.219, 0.079 and 0.329 ng/mL, respectively. At the same time, it also showed strong specificity. The strip was stable and could be stored at room temperature for up to one year, and the average recovery rate was 85.98–115.92%. In addition, the EuNPs−FIA was validated by high-performance liquid chromatography (HPLC) analysis, and a satisfactory consistency was obtained. Compared to the current immunochromatographic methods used for detecting abused drugs in hair, this method not only increased the number of detection targets, but also ensured sensitivity, improving detection efficiency to a certain extent. The approach can also be used as an alternative to chromatography. It provides a rapid and accurate screening method for the detection of abused drugs in hair and has great application prospects in regard to public safety.

show abstract

Fluorescent paper strip immunoassay with carbon nanodots@silica for determination of human serum amyloid A1

Cited by 9 publications

References 37 publications

Highly Sensitive and Ultrastable Lateral Flow Immunoassay Based on Polydopamine-Coated Aggregation-Induced Emission Fluorescent Microspheres with Excellent Fluorescence Performance and Biofriendly Coupling Strategy

Highly Sensitive and Ultrastable Lateral Flow Immunoassay Based on Polydopamine-Coated Aggregation-Induced Emission Fluorescent Microspheres with Excellent Fluorescence Performance and Biofriendly Coupling Strategy

Nanomaterial and Interface Advances in Immunoassay Biosensors

Europium Nanoparticles-Based Fluorescence Immunochromatographic Detection of Three Abused Drugs in Hair

Contact Info

Product

Resources

About