Fluorescent oligonucleotide indicators for ratiometric microRNA sensing on metal-organic frameworks

Wang, Yihui; Shao, Zhen-Shu; Chen, Cheng; Wang, Jiali; Song, Zhen; Song, Weijie; Zheng, Feng; Wang, Huai-Song

doi:10.1016/j.cej.2022.135296

Cited by 25 publications

(12 citation statements)

References 49 publications

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“…These biomolecules include amino acids, 128 peptides, 129 proteins, 130 etc. , 131 and the body fluids which were commonly detected include blood, 132 urine, 133 sweat, 134 tears, 135 etc. These body fluids contain multiple kinds of biomolecules which interfere with the detection of targeted biomolecules.…”

Section: Application Of Aunc Compositesmentioning

confidence: 99%

Gold nanocluster composites: preparation strategies, optical and catalytic properties, and applications

Zhu

Weng

et al. 2022

J. Mater. Chem. C

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Section: Application Of Aunc Compositesmentioning

confidence: 99%

Gold nanocluster composites: preparation strategies, optical and catalytic properties, and applications

Zhu

Weng

et al. 2022

J. Mater. Chem. C

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“…19,20 Recently, MOFs show unique capabilities in the field of DNA sensing, 21 usually with a DNA fluorescent probe (or aptamer). 22 The interaction between fluorescent probes and MOFs mainly depends on π−π interaction, hydrogen bonding, and electrostatic forces. 23 The internal fluorescence quenching mechanisms are ascribed to PET 24 and FRET.…”

Section: Introductionmentioning

confidence: 99%

“…Metal–organic frameworks (MOFs) are porous crystalline materials composed of metal clusters and organic ligands. , Recently, MOFs show unique capabilities in the field of DNA sensing, usually with a DNA fluorescent probe (or aptamer) . The interaction between fluorescent probes and MOFs mainly depends on π–π interaction, hydrogen bonding, and electrostatic forces .…”

Section: Introductionmentioning

confidence: 99%

Rapid and Ultrasensitive Fluorescence Sensing Platform Based on Nanometer-Sized Metal–Organic Frameworks for Transgenic CaMV 35S Promoter Detection

Liu

Zhou

Dong

et al. 2023

ACS Appl. Nano Mater.

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Rapid and sensitive identification of genetically modified organisms (GMOs) is urgent for food safety and people health. Herein, a rapid and ultrasensitive fluorescence sensing platform is developed based on the single-strand DNA-labeled FAM dye (FAM-ssDNA) and nanometer-sized Fe-metal–organic framework (Fe-MOF), Fe-MIL-88. Fe-MIL-88 possesses a large specific surface area, remarkable preferential combining capacity with ssDNA versus dsDNA, and great quenching ability. FAM-ssDNA probes are elaborately designed as facile fluorescent probes to be fully complementary with the target of cauliflower mosaic virus 35S promoter (CaMV 35S), which can be absorbed to the surface of Fe-MIL-88 through π–π stacking and electrostatic interaction. The results indicate that the quenching ability of Fe-MIL-88 is attributed to the combination of photoinduced electron transfer (PET) and fluorescence resonance energy transfer (FRET) processes. Benefiting from the distinguishable ability of Fe-MIL-88, the fluorescent biosensor exhibits a satisfactory linear range from 5 pM to 50 nM and a low limit of detection (LOD) down to 0.184 pM. Also, the operation time was less than 1 h. Furthermore, the sensor also performs well in the anti-interference test and real sample test. Importantly, the sensing platform is equipped with easier operation without any complicated and difficult immobilization steps. Therefore, the sensor owns cheering potential for the detection of GMOs.

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“…Sensitive detection of miRNAs remains difficult because of their low expression and high sequence similarity . Therefore, some signal amplification strategies such as nicking enzyme-assisted amplification, DSN-assisted amplification, rolling circle amplification, catalytic hairpin assembly, and hybridization chain reaction have been introduced to improve the sensitivity. − Among them, DSN-based miRNA biosensing is regarded as a promising strategy with the merits of high sensitivity and specificity.…”

Section: Introductionmentioning

confidence: 99%

Ratiometric Fluorescent Biosensor Based on Self-Assembled Fluorescent Gold Nanoparticles and Duplex-Specific Nuclease-Assisted Signal Amplification for Sensitive Detection of Exosomal miRNA

Sun

Yang

et al. 2022

Bioconjugate Chem.

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The sensitive detection of cancer-associated exosomal microRNAs shows enormous potential in cancer diagnosis. Herein, a ratiometric fluorescent biosensor based on self-assembled fluorescent gold nanoparticles (Au NPs) and duplex-specific nuclease (DSN)-assisted signal amplification was fabricated for sensitive detection of colorectal cancer (CRC)-associated exosomal miR-92a-3p. In this biosensing system, the hairpin DNA modified with sulfhydryl and fluorescent dye Atto-425 at both ends is conjugated to fluorescent Au NPs through Au–S bonds, resulting in the quenching of Atto-425. The miR-92a-3p can open the hairpin of DNA and forms an miR-92a-3p/DNA heteroduplex, triggering the specific cleavage of DSN for the DNA in the heteroduplex. As a result, Atto-425 leaves the fluorescent Au NPs and recovers the fluorescence emission. The released miR-92a-3p can hybridize with another hairpin DNA and lead to a stronger fluorescence recovery of Atto-425 to form a signal amplification cycle. The stable fluorescence of Au NPs and the changing fluorescence of Atto-425 constitute a ratiometric fluorescent system reflecting the concentration of miR-92a-3p. This biosensor exhibits excellent specificity and can distinguish CRC patients from healthy individuals by detecting miR-92a-3p extracted from clinical exosome samples, showing the potential in CRC diagnosis.

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Fluorescent oligonucleotide indicators for ratiometric microRNA sensing on metal-organic frameworks

Cited by 25 publications

References 49 publications

Gold nanocluster composites: preparation strategies, optical and catalytic properties, and applications

Gold nanocluster composites: preparation strategies, optical and catalytic properties, and applications

Rapid and Ultrasensitive Fluorescence Sensing Platform Based on Nanometer-Sized Metal–Organic Frameworks for Transgenic CaMV 35S Promoter Detection

Ratiometric Fluorescent Biosensor Based on Self-Assembled Fluorescent Gold Nanoparticles and Duplex-Specific Nuclease-Assisted Signal Amplification for Sensitive Detection of Exosomal miRNA

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