Fluorescent nanosensor designing via hybrid of carbon dots and post-imprinted polymers for the detection of ovalbumin

Abstract

We reported a facile strategy to assemble a ratiometric nanosensor for the ovalbumin (OVA) fluorescence determination and meanwhile it can be utilized for selective visual identification by naked eyes with fluorescent test papers under 365 nm UV lamp. The nanosensor was prepared through simply mixing blue color carbon dots (CDs) and green color core-shell imprinted polymers. Blue CDs were used directly as the internal reference without participating in the imprinting process and modified molecularly imprinted polymers (MIPs) were synthesized by post-imprinting, using fluorescein isothiocyanate (FITC) as fluorescence enhanced signal. Upon the addition of different concentrations of OVA, the fluorescence intensity of FITC was enhanced, while the fluorescence intensity of CDs was almost unchanged, leading to a detection limit as low as 15.4 nM. Accordingly, the fluorescence color was gradually changed from blue to dark olive green to green with naked eyes observation. Moreover, the ratiometric nanosensor was successfully applied to detect OVA in the human urine samples with satisfactory recoveries attaining of 92.0-104.0% with relative standard deviation (RSD) of 3.3-3.9% and 93.3-101.0% with RSDs of 2.7-3.8% for the spiked chicken egg white samples. This strategy reported here opens a novel pathway for biomacromolecule detection in real applications and can realize the visual observation on fluorescent test papers.

Fluorescence Sensing Performance of Carbon Dots of Functionalization toward Sunset Yellow

“…This indicates that there is no IFE between CDs‐PTD and FCF, because there is almost no change in the fluorescence lifetime after the quencher is added during IFE. [ 46,47 ] Based on the above description, the fluorescence quenching mechanism of FCF to CDs‐PTD is FRET and the response mechanism is schematically shown in Figure 6a. The FRET mechanism is only the transfer of energy does not involve the transfer of photons.…”

Effect of polysaccharides on the functional properties of egg white protein: A review

“…Therefore, an in‐depth understanding of the self‐assembly aggregation mechanism of protein and polysaccharide composite gels would be the focus to be discussed in the next step. For example, a nanosensor for OVA fluorescence determination can be used to detect OVA binding form based on visual observation (Wang, Yu et al., 2020). At present, most protein‐polysaccharide gels were in the laboratory stage, but large‐scale composite gel preparation methods need to be set up.…”

Complete and Versatile Post‐Synthetic Modification on Iron‐Triazole Spin Crossover Complexes: A Relevant Material Elaboration Method

“…In recent years the post-synthetic modification (PSM) method has attracted more attention as an efficient tool to perform a chemical transformation on previously synthesized materials such as metal-organic frameworks (MOFs), [1][2][3][4] covalent organic frameworks (COFs), [5] quantum dots, [6] carbon dot, [7] biomolecules, [8][9][10][11] polymers, [12] supramolecular assemblies. [13,14] The term "post-synthetic modification" was first introduced by Cohen and Wang [15] in the PSM of MOFs as an analogy to protein post-translational modification (PTM).…”