Fluorescent nanodiamonds for specifically targeted bioimaging: Application to the interaction of transferrin with transferrin receptor

Weng, Mao Feng; Chiang, Su‐Yu; Wang, Niann‐Shiah; Niu, Huan

doi:10.1016/j.diamond.2008.07.012

Cited by 69 publications

(51 citation statements)

References 30 publications

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“…Notably, the ND always bears a mixture of various moieties because the reactions never reach 100 % completion grafting, on the other hand, creates a stronger, much more specific connection between a molecule and the ND surface (Huang and Chang 2004). These connections are usually realized via amide bonds; either an amino group of a biomolecule reacts with a carboxyl group on the ND surface (Dahoumane et al 2009;Sreenivasan et al 2011;Fu et al 2007Fu et al , 2012Cheng et al 2007;Wei et al 2010;Zhang et al 2009a;Mkandawire et al 2009;Mohan et al 2010;Li and Zhou 2010;Weng et al 2009Weng et al , 2012 or vice versa (Hens et al 2008). Silane linkers can be also used to connect biomolecules to the ND surface by forming a covalent amide bond (Krüger et al 2006;Krueger et al 2008) or by the reaction of isocyanate with an amino group (Martín et al 2010a).…”

Section: Biomolecules On the Nd Surfacementioning

confidence: 98%

Nanodiamonds as Intracellular Probes for Imaging in Biology and Medicine

Šlegerová

Řehoř

Havlík

et al. 2014

Fundamental Biomedical Technologies

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In recent years, diamond nanoparticles have received a great deal of attention due to their unique photophysical and biological properties. Nanodiamonds (NDs) show low toxicity and are considered to be a highly biocompatible carbon nanomaterial useful in a wide range of applications. Thanks to their ability to accommodate nitrogen-vacancy (N-V) color centers, NDs are a prime example of non-photobleachable fluorescent labels and nanosensors. Here, we present a survey of ND applications in biology and medicine with an emphasis on bioimaging. We focus on distinguishing the properties of detonation NDs and highpressure high-temperature (HPHT) NDs and describing their physicochemical properties, structure and possible modifications by small molecules and biomolecules. We summarize and critically evaluate in vitro and in vivo data on ND toxicity and biocompatibility, cellular internalization, localization and targeting by surface-attached ligands. We discuss current achievements in bioimaging using fluorescent NDs and the potential of NDs in diagnostics and drug delivery.

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Section: Biomolecules On the Nd Surfacementioning

confidence: 98%

Nanodiamonds as Intracellular Probes for Imaging in Biology and Medicine

Šlegerová

Řehoř

Havlík

et al. 2014

Fundamental Biomedical Technologies

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“…However, nanodiamond size, conjugated functional groups, solution and cell type all influence the process. For example, Weng et al (2009) found that transferrin-conjugated 100-nm diamonds were taken up via the abundant transferrin receptors on HeLa cells in a clathrin-dependant manner. In contrast, Cheng et al (2007) observed that similar-sized nanodiamonds conjugated to growth hormone remained extracellular, presumably attached to the binding domain of the growth hormone receptor on A549 cells, while the unconjugated control nanodiamonds were internalised.…”

Section: Bio-compatibility Of Nanodiamondmentioning

confidence: 99%

“…3) (Fu et al 2007;Chang et al 2008b;Zhang et al 2009a) more recently in real time (Faklaris et al 2009a) using single particle tracking within cells. As nanodiamonds do not enter the nucleus Chang et al 2008a, b;Wee et al 2009;Zhang et al 2009a) and because their bright fluorescence is emitted beyond the range of cellular autofluorescence Fu et al 2007;Fucikova et al 2009;Weng et al 2009), they can be used as cytoskeletal markers at concentrations as low as 1 μg/mL and detected using confocal fluorescence microscopy ). Furthermore, because of the long radiative lifetime of NV colour centres (approx.…”

Section: Detection Imaging and Tracking In Vitromentioning

confidence: 99%

Luminescent nanodiamonds for biomedical applications

et al. 2011

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In recent years, nanodiamonds have emerged from primarily an industrial and mechanical applications base, to potentially underpinning sophisticated new technologies in biomedical and quantum science. Nanodiamonds are relatively inexpensive, biocompatible, easy to surface functionalise and optically stable. This combination of physical properties are ideally suited to biological applications, including intracellular labelling and tracking, extracellular drug delivery and adsorptive detection of bioactive molecules. Here we describe some of the methods and challenges for processing nanodiamond materials, detection schemes and some of the leading applications currently under investigation.

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“…123 In addition to resistance to photobleaching, the fluorescence of NDs (35 and 100 nm in size) was also found to be immune to photoblinking on a time scale of 1 millisecond. 25 Moreover, due to the deep location of (N-V) -centers in the lattice structure of NDs, 124,125 the fluorescence remains unaffected by surface alterations, as evidenced by NDs functionalized with carboxylic acid, 69 polylysine, 25 and transferrin 126 groups. Therefore, the intrinsic photostability of NDs opens avenues for carrying out chemical modification of their surface for specific imaging applications.…”

Section: Nds As Bioimaging Agents Fluorescence Of the Ndsmentioning

confidence: 99%

“…126 They oxidized 100 nm red fluorescent NDs and conjugated transferrin to their surfaces through an amide linkage to form ND-transferrin conjugates. 126 These bioconjugates were then incubated for an hour at a concentration of 10 µg/mL with HeLa cells overexpressing transferrin receptors.…”

Section: Nds As Bioimaging Agents Fluorescence Of the Ndsmentioning

confidence: 99%

Nanodiamonds as novel nanomaterials for biomedical applications: drug delivery and imaging systems

Kaur¹,

Badea²

2013

IJN

103

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Detonation nanodiamonds (NDs) are emerging as delivery vehicles for small chemical drugs and macromolecular biotechnology products due to their primary particle size of 4 to 5 nm, stable inert core, reactive surface, and ability to form hydrogels. Nanoprobe technology capitalizes on the intrinsic fluorescence, high refractive index, and unique Raman signal of the NDs, rendering them attractive for in vitro and in vivo imaging applications. This review provides a brief introduction of the various types of NDs and describes the development of procedures that have led to stable single-digit-sized ND dispersions, a crucial feature for drug delivery systems and nanoprobes. Various approaches used for functionalizing the surface of NDs are highlighted, along with a discussion of their biocompatibility status. The utilization of NDs to provide sustained release and improve the dispersion of hydrophobic molecules, of which chemotherapeutic drugs are the most investigated, is described. The prospects of improving the intracellular delivery of nucleic acids by using NDs as a platform are exemplified. The photoluminescent and optical scattering properties of NDs, together with their applications in cellular labeling, are also reviewed. Considering the progress that has been made in understanding the properties of NDs, they can be envisioned as highly efficient drug delivery and imaging biomaterials for use in animals and humans.

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Fluorescent nanodiamonds for specifically targeted bioimaging: Application to the interaction of transferrin with transferrin receptor

Cited by 69 publications

References 30 publications

Nanodiamonds as Intracellular Probes for Imaging in Biology and Medicine

Nanodiamonds as Intracellular Probes for Imaging in Biology and Medicine

Luminescent nanodiamonds for biomedical applications

Nanodiamonds as novel nanomaterials for biomedical applications: drug delivery and imaging systems

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