Fluorescent molecularly imprinted polymer studied by time-resolved fluorescence spectroscopy

Wandelt, Barbara; Turkewitsch, Petra; Wysocki, S.; Darling, Graham D.

doi:10.1016/s0032-3861(02)00066-6

Cited by 25 publications

(18 citation statements)

References 23 publications

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“…108,[112][113][114][115][116] Normally in these systems, recognition of the analytes results in the quenching of the fluorescence emission. However, in sensor design enhancement of the signal is more desirable.…”

Section: Fluorescent Polymersmentioning

confidence: 99%

Design of fluorescent materials for chemical sensing

2007

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There is an enormous demand for chemical sensors for many areas and disciplines. High sensitivity and ease of operation are two main issues for sensor development. Fluorescence techniques can easily fulfill these requirements and therefore fluorescent-based sensors appear as one of the most promising candidates for chemical sensing. However, the development of sensors is not trivial; material science, molecular recognition and device implementation are some of the aspects that play a role in the design of sensors. The development of fluorescent sensing materials is increasingly captivating the attention of the scientists because its implementation as a truly sensory system is straightforward. This critical review shows the use of polymers, sol-gels, mesoporous materials, surfactant aggregates, quantum dots, and glass or gold surfaces, combined with different chemical approaches for the development of fluorescent sensing materials. Representative examples have been selected and they are commented here.

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Section: Fluorescent Polymersmentioning

confidence: 99%

Design of fluorescent materials for chemical sensing

2007

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“…the ratio of useful binding sites to the total number of imprinted binding sites) and uniform binding sites (12). To examine MIP's binding performance, one often relies on the fluorescence signals of bound analytes (13) or polymers (14). Steady-state fluorescence reveals the presence of analytes but lacks detailed information about the local binding environments.…”

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confidence: 99%

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“…Few research groups have studied the timeresolved fluorescence of MIPs (14,17). Wandelt et al incorporated fluorescent monomers into cAMPimprinted polymers and detected the quenching of fluorescence of the polymer itself as the cAMP bound with the MIPs (14). Their results showed different fluorescence lifetime distributions between specific and non-specific bindings; this suggests that timeresolved fluorescence measurements could possibly be used to characterize the binding specificity of MIPs.…”

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confidence: 99%

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Fluorescence anisotropy studies of molecularly imprinted polymers

et al. 2005

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A molecularly imprinted polymer (MIP) is a biomimetic material that can be used as a biochemical sensing element. We studied the steady-state and time-resolved fluorescence and fluorescence anisotropy of anthracene-imprinted polyurethane. We compared MIPs with imprinted analytes present, MIPs with the imprinted analytes extracted, MIPs with rebound analytes, non-imprinted control polymers (non-MIPs) and non-MIPs bound with analytes to understand MIP's binding behaviour. MIPs and non-MIPs had similar steady-state fluorescence anisotropy in the range 0.11-0.24. Anthracene rebound in MIPs and non-MIPs had a fluorescence lifetime of tau = 0.64 ns and a rotational correlation time of phi(F) = 1.2-1.5 ns, both of which were shorter than that of MIPs with imprinted analytes present (tau = 2.03 ns and phi(F) = 2.7 ns). The steady-state anisotropy of polymer solutions increased exponentially with polymerization time and might be used to characterize the polymerization extent in situ.

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“…In a different approach, a functional monomer can be derivatized to include, for instance, a fluorescent moiety that changes its optical properties upon re-binding of the analyte [4,11]. In fact, the study of the changes in the spectroscopic and photophysical properties of the reporter molecule in the presence of the polymer can be of great help in the characterization of the binding sites of the material [12,13]. Zearalenone (ZON.…”

Section: Introductionmentioning

confidence: 99%