Fluorescent Minerals

Modreski, Peter J.; Aumente-Modreski, R. M.

doi:10.1080/00357529.1996.11761532

Cited by 8 publications

(8 citation statements)

References 11 publications

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“…Having assumed that snail uses physical-and cellular-based processes [87][88][89][90] in shell regeneration, we aimed to analyze the polymorphs of the crystals structures obtained by biomineralization ex vivo. Additional analysis of the obtained deposits carried out by fluorescence microscopy indicated similarity between calcite standard [92,93] and microgranular phase formed after biomineralization ex vivo using C. aspersum hemolymph with respect to the red auto-fluorescence (see Fig. 11).…”

Section: Resultsmentioning

confidence: 88%

Electrochemical method for isolation of chitinous 3D scaffolds from cultivated Aplysina aerophoba marine demosponge and its biomimetic application

et al. 2020

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Three-dimensional (3D) biopolymer-based scaffolds including chitinous matrices have been widely used for tissue engineering, regenerative medicine and other modern interdisciplinary fields including extreme biomimetics. In this study, we introduce a novel, electrochemically assisted method for 3D chitin scaffolds isolation from the cultivated marine demosponge Aplysina aerophoba which consists of three main steps: (1) decellularization, (2) decalcification and (3) main deproteinization along with desilicification and depigmentation. For the first time, the obtained electrochemically isolated 3D chitinous scaffolds have been further biomineralized ex vivo using hemolymph of Cornu aspersum edible snail aimed to generate calcium carbonates-based layered biomimetic scaffolds. The analysis of prior to, during and post-electrochemical isolation samples as well as samples treated with molluscan hemolymph was conducted employing analytical techniques such as SEM, XRD, ATR-FTIR and Raman spectroscopy. Finally, the use of described method for chitin isolation combined with biomineralization ex vivo resulted in the formation of crystalline (calcite) calcium carbonate-based deposits on the surface of chitinous scaffolds, which could serve as promising biomaterials for the wide range of biomedical, environmental and biomimetic applications.

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Section: Resultsmentioning

confidence: 88%

Electrochemical method for isolation of chitinous 3D scaffolds from cultivated Aplysina aerophoba marine demosponge and its biomimetic application

et al. 2020

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“…We assumed that calcite was likely to be the biomineral formed ex vivo by hemocytes on the surface of 3D chitinous scaffolds ( Figure 9C,D), and so we carried out the initial analysis of deposits, using fluorescence microscopy. The results obtained ( Figure 10) clearly show a high similarity of red auto-fluorescence between calcite standard [114,115] and microgranular deposits formed after biomineralization ex vivo, using C. aspersum hemolymph. To analyze the obtained biomineral with respect to calcite composition, we used traditional analytical techniques, such as X-ray diffraction analysis (XRD) (Figure 11), FTIR ( Figure 12) and Raman (Figure 13) spectroscopy.…”

Section: Figurementioning

confidence: 76%

3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo

et al. 2020

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Structure-based tissue engineering requires large-scale 3D cell/tissue manufacture technologies, to produce biologically active scaffolds. Special attention is currently paid to naturally pre-designed scaffolds found in skeletons of marine sponges, which represent a renewable resource of biomaterials. Here, an innovative approach to the production of mineralized scaffolds of natural origin is proposed. For the first time, a method to obtain calcium carbonate deposition ex vivo, using living mollusks hemolymph and a marine-sponge-derived template, is specifically described. For this purpose, the marine sponge Aplysin aarcheri and the terrestrial snail Cornu aspersum were selected as appropriate 3D chitinous scaffold and as hemolymph donor, respectively. The formation of calcium-based phase on the surface of chitinous matrix after its immersion into hemolymph was confirmed by Alizarin Red staining. A direct role of mollusks hemocytes is proposed in the creation of fine-tuned microenvironment necessary for calcification ex vivo. The X-ray diffraction pattern of the sample showed a high CaCO3 amorphous content. Raman spectroscopy evidenced also a crystalline component, with spectra corresponding to biogenic calcite. This study resulted in the development of a new biomimetic product based on ex vivo synthetized ACC and calcite tightly bound to the surface of 3D sponge chitin structure.

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“…A previous study using remote laser-induced fluorescence to investigate various soils and rocks up to ~100,000 years old found that fluorescence in rocks such as the matrix around a fossil could be largely attributed to residual organic material 27 . It is also known that minerals can also fluoresce, especially if they contain impurities in their crystal structure, which could be an additional source of fluorescence from the fossil matrix 28 , 29 . Figure 1 illustrates that the emission spectra within certain regions of the leaf exhibit a partial overlap with the spectrum observed in the matrix region.…”

Section: Resultsmentioning

confidence: 99%

Two-photon excitation fluorescence microspectroscopy protocols for examining fluorophores in fossil plants

Stoneman,

McCoy,

Gee

et al. 2024

Commun Biol

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Fluorescence emission is common in plants. While fluorescence microscopy has been widely used to study living plants, its application in quantifying the fluorescence of fossil plants has been limited. Fossil plant fluorescence, from original fluorophores or formed during fossilization, can offer valuable insights into fluorescence in ancient plants and fossilization processes. In this work, we utilize two-photon fluorescence microspectroscopy to spatially and spectrally resolve the fluorescence emitted by amber-embedded plants, leaf compressions, and silicified wood. The advanced micro-spectroscope utilized, with its pixel-level spectral resolution and line-scan excitation capabilities, allows us to collect comprehensive excitation and emission spectra with high sensitivity and minimal laser damage to the specimens. By applying linear spectral unmixing to the spectrally resolved fluorescence images, we can differentiate between (a) the matrix and (b) the materials that comprise the fossil. Our analysis suggests that the latter correspond to durable tissues such as lignin and cellulose. Additionally, we observe potential signals from chlorophyll derivatives/tannins, although minerals may have contributed to this. This research opens doors to exploring ancient ecosystems and understanding the ecological roles of fluorescence in plants throughout time. Furthermore, the protocols developed herein can also be applied to analyze non-plant fossils and biological specimens.

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Fluorescent Minerals

Cited by 8 publications

References 11 publications

Electrochemical method for isolation of chitinous 3D scaffolds from cultivated Aplysina aerophoba marine demosponge and its biomimetic application

Electrochemical method for isolation of chitinous 3D scaffolds from cultivated Aplysina aerophoba marine demosponge and its biomimetic application

3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo

Two-photon excitation fluorescence microspectroscopy protocols for examining fluorophores in fossil plants

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