Fluorescent microsphere probe for rapid qualitative and quantitative detection of trypsin activity

Abstract

The fluorescent PS-PEG-peptide-FITC microsphere probe was designed for real-time, qualitative and quantitative monitoring of trypsin activity.

Biomimetic NIR-II fluorescent proteins created from chemogenic protein-seeking dyes for multicolor deep-tissue bioimaging

“…This strategy is universal and applicable in creating a wide range of spectrally separated NIR-I/II FPs for real-time visualization of multiple biological events. Overall, this straightforward biomimetic approach holds the potential to transform fluorescent protein-based bioimaging and enables in-situ albumin targeting to create NIR-I/II FPs for deep-tissue imaging in live organisms.Fluorescence imaging has received extensive attention in diverse multidisciplinary fields as a cutting-edge imaging technology due to its high sensitivity, excellent spatial-temporal resolution, and strong specificity [1][2][3][4][5][6][7] . Although it has been successful in unraveling the intricacies of biological systems, intravital deep-tissue imaging remains a significant challenge due to the optical opacity of biological tissues 8,9 .…”

“…Fluorescence imaging has received extensive attention in diverse multidisciplinary fields as a cutting-edge imaging technology due to its high sensitivity, excellent spatial-temporal resolution, and strong specificity [1][2][3][4][5][6][7] . Although it has been successful in unraveling the intricacies of biological systems, intravital deep-tissue imaging remains a significant challenge due to the optical opacity of biological tissues 8,9 .…”