Fluorescent Microscopic Analysis of Mandibula Surrounding a Pair of Titanium Implants as Anchorage for Orthodontic Tooth Movement in Adult Male Beagle Dogs.

Abstract

Abstract:The aim of the present study was to determine the anchorage potential of Branemark titanium implants for orthodontic mesio-distal tooth movement in four adult male Beagle do gs. Two implants were surgically placed bilaterally in healed mandibular extraction sites of the second and third premolars on each side. After 18 weeks, full-casted metal crowns were fitted to the pair of implant s and the fourth premolar (P4). Arch wires with a T-loop or an L-loop were placed between the implants and P4 . The arch wire was calibrated to produce 200g of lateral force on P4 (loaded side) or none ( unloaded side). Tetracycline was injected subcutaneously biweekly . The animals were killed at weeks 24 , 28, 28, and 32, and dissected mandibles were prepared for backscattered electron images and fluorescence microscopic observation . Trimmed mandibula with implants were sagittally-sectioned , and then cross-sectioned in half . The percentages of calcified area (Cal Area) and tetracycline-labeled area (TC Area) were calculated from the region between the cross-sectioned implant surface and a line 0 .5mm from the surface on the mesial or distal sid e. P4on the loaded side moved 3 to 10mm mesially , while that on the unloaded side did not move . The back scattered electron image from the sagittal section on the loaded side was simila r to that on the unloaded side . No significant diff erences in either Cal Area (ave . 80%) or TC Area (ave . 35%) were noted between the control and experimental sides . These results indicate that endosseous titanium i mplants connected to a metal crown can function as anchors for orthodontic tooth movement .