Fluorescent metabolic labeling-based quick antibiotic susceptibility test for anaerobic bacteria

Gao, Juan; Qin, Juanxiu; Ding, Chenling; Gao, Yuan; Guo, Junnan; Li, Min; Yang, Chaoyong; Wang, Wei

doi:10.1039/d2cb00163b

“…As the metabolic activity of bacteria can be quantified through the metabolic labeling method, it has been applied to monitor the effects of antibiotics on the metabolic activity of bacteria, thereby determining the sensitivity and minimum inhibitory concentration (MIC) of bacteria to antibiotics. [47]…”

Section: Metabolic Activities and Viability Of Bacteriamentioning

confidence: 99%

Development and Applications of D‐Amino Acid Derivatives‐based Metabolic Labeling of Bacterial Peptidoglycan

Zheng,

Zhu,

Jiang

et al. 2024

Angewandte Chemie

0

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Peptidoglycan, an essential component within the cell walls of virtually all bacteria, is composed of glycan strands linked by stem peptides that contain D‐amino acids. The peptidoglycan biosynthesis machinery exhibits high tolerance to various D‐amino acid derivatives. D‐amino acid derivatives with different functionalities can thus be specifically incorporated into and label the peptidoglycan of bacteria, but not the host mammalian cells. This metabolic labeling strategy is highly selective, highly biocompatible, and broadly applicable, which has been utilized in various fields. This review introduces the metabolic labeling strategies of peptidoglycan by using D‐amino acid derivatives, including one‐step and two‐step strategies. In addition, we emphasize the various applications of D‐amino acid derivative‐based metabolic labeling, including bacterial peptidoglycan visualization (existence, biosynthesis, and dynamics, etc.), bacterial visualization (including bacterial imaging and visualization of growth and division, metabolic activity, antibiotic susceptibility, etc.), pathogenic bacteria‐targeted diagnostics and treatment (positron emission tomography (PET) imaging, photodynamic therapy, photothermal therapy, gas therapy, immunotherapy, etc.), and live bacteria‐based therapy. Finally, a summary of this metabolic labeling and an outlook is provided.

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Development and Applications of D‐Amino Acid Derivatives‐based Metabolic Labeling of Bacterial Peptidoglycan

Zheng,

Zhu,

Jiang

et al. 2024

Angew Chem Int Ed

1

0

View full text Add to dashboard Cite

Peptidoglycan, an essential component within the cell walls of virtually all bacteria, is composed of glycan strands linked by stem peptides that contain D‐amino acids. The peptidoglycan biosynthesis machinery exhibits high tolerance to various D‐amino acid derivatives. D‐amino acid derivatives with different functionalities can thus be specifically incorporated into and label the peptidoglycan of bacteria, but not the host mammalian cells. This metabolic labeling strategy is highly selective, highly biocompatible, and broadly applicable, which has been utilized in various fields. This review introduces the metabolic labeling strategies of peptidoglycan by using D‐amino acid derivatives, including one‐step and two‐step strategies. In addition, we emphasize the various applications of D‐amino acid derivative‐based metabolic labeling, including bacterial peptidoglycan visualization (existence, biosynthesis, and dynamics, etc.), bacterial visualization (including bacterial imaging and visualization of growth and division, metabolic activity, antibiotic susceptibility, etc.), pathogenic bacteria‐targeted diagnostics and treatment (positron emission tomography (PET) imaging, photodynamic therapy, photothermal therapy, gas therapy, immunotherapy, etc.), and live bacteria‐based therapy. Finally, a summary of this metabolic labeling and an outlook is provided.

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Metabolic Labeling and Digital Microfluidic Single‐Cell Sequencing for Single Bacterial Genotypic‐Phenotypic Analysis

Guo,

Sun,

Li

et al. 2024

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Accurate assessment of phenotypic and genotypic characteristics of bacteria can facilitate comprehensive cataloguing of all the resistance factors for better understanding of antibiotic resistance. However, current methods primarily focus on individual phenotypic or genotypic profiles across different colonies. Here, a Digital microfluidic‐based automated assay for whole‐genome sequencing of single‐antibiotic‐resistant bacteria is reported, enabling Genotypic and Phenotypic Analysis of antibiotic‐resistant strains (Digital‐GPA). Digital‐GPA can efficiently isolate and sequence antibiotic‐resistant bacteria illuminated by fluorescent D‐amino acid (FDAA)‐labeling, producing high‐quality single‐cell amplified genomes (SAGs). This enables identifications of both minor and major mutations, pinpointing substrains with distinctive resistance mechanisms. Digital‐GPA can directly process clinical samples to detect and sequence resistant pathogens without bacterial culture, subsequently provide genetic profiles of antibiotic susceptibility, promising to expedite the analysis of hard‐to‐culture or slow‐growing bacteria. Overall, Digital‐GPA opens a new avenue for antibiotic resistance analysis by providing accurate and comprehensive molecular profiles of antibiotic resistance at single‐cell resolution.

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Fluorescent metabolic labeling-based quick antibiotic susceptibility test for anaerobic bacteria

Abstract: Via a fluorescent d-amino acid (FDAA) labeling-based antibiotic susceptibility test method, the minimum inhibitory concentrations of anaerobic bacteria were accurately determined in ∼5 h.

Cited by 3 publications

References 27 publications

Development and Applications of D‐Amino Acid Derivatives‐based Metabolic Labeling of Bacterial Peptidoglycan

Development and Applications of D‐Amino Acid Derivatives‐based Metabolic Labeling of Bacterial Peptidoglycan

Development and Applications of D‐Amino Acid Derivatives‐based Metabolic Labeling of Bacterial Peptidoglycan

Metabolic Labeling and Digital Microfluidic Single‐Cell Sequencing for Single Bacterial Genotypic‐Phenotypic Analysis

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