Fluorescent magnetic nanoparticles for modulating the level of intracellular Ca²⁺ in motoneurons

Abstract

This report introduces both synthesis and in vitro biological behaviour of dual magnetic-fluorescent silica nanoparticles.

Determination of the viscosity of the cytoplasm of M-HeLa cells using fluorescent magnetic nanoparticles and an electromagnetic needle

“…In the next series of experiments, using EN, we studied the effect of the magnetic eld gradient on MNPs loaded into HeLa cancer cells. Cells in culture were loaded with nanoparticles according to the method described by S. Fedorenko (Fedorenko et al 2019). As a result, 100 percent of the cells in culture were internalized with MNPs (Fig.…”

“…In the present study, superparamagnetic SPION NPs with an iron oxide core and a uorescent complex [Ru(dipy) 3 ] 2+ are used. The MNP data was synthesized in accordance with the previously published procedure (Fedorenko et al 2019). These are amino-modi ed silica nanoparticles (78 nm in diameter) doped with iron oxide cores.…”

“…The encapsulation of [Ru(dipy) 3 ] 2+ phosphorescent complexes into a silica matrix which allows to control the penetration of the MNPs into cells and to monitor the behavior of the MNPs inside cells using a confocal uorescent microscope due to the bright emission in the red wavelength range upon the excitation (450 nm). The magnetic properties of the MNPs are ensured by the inclusion of superparamagnetic iron oxide cores 17.1 nm in size in the silica matrix (Fedorenko et al 2019).…”

Luminescent Composite Carbon/SiO2 Structures: Synthesis and Applications

“…Silica structures are often used as a matrix for nanoparticles [ 35 , 66 , 67 , 68 , 69 ] due to their thermodynamic and chemical stability in various chemical environments, low toxicity, biodegradability, optical transparency, and high surface area [ 34 , 69 , 70 ]. The presence of hydroxyl groups on the silica surface leads to high hydrophilicity and contributes to further functionalization [ 34 ].…”

“…The literature reports various examples of matrices such as silica [ 18 , 19 , 20 , 21 , 22 , 23 ], polymers [ 24 , 25 , 26 ], starch [ 27 ], zeolites [ 28 , 29 , 30 ], potassium aluminum sulfate [ 31 ], trisodium citrate [ 32 ], and sodium chloride crystals [ 33 ]. Currently, silica is a powerful and effective matrix thanks to its optical transparency [ 34 , 35 ], biocompatibility [ 34 ], low toxicity [ 34 , 36 ], tunable dielectric properties [ 37 ], and potential for size and surface customization [ 34 , 36 ].…”