Fluorescent Ligands of Kv1 Channels on the Basis of Hongotoxin: Atto488-Hongotoxin

Orlov, Nikita A.; Nekrasova, Oksana V.; Feofanov, Аlexey V.

doi:10.1017/s1431927619007128

Cited by 6 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to visualising native channels, venom toxins were used also for imaging chimeric K + channel proteins (KcsA-K V 1,x; x=1,3,6) made by transfer of the eukaryotic (K V ) S5-S6 linker region within the pore domain in bacterial homologues (KcsA) [ 67 ]. The specific binding of ATTO488-labelled HgTx [ 59 ] and TAMRA-tagged AgTx-2 [ 60 ] with KcsA-K V 1.1 and KcsA-K V 1.3 chimaeras expressed in E. coli spheroplasts enabled their visualisation using confocal imaging. Likewise, in E. coli with and without recombinant KcsA-K V 1.x (1,3,6), OSK1-eGFP (toxin derived from scorpion Orthochirus scrobiculosus ) and AgTx-2-TagRFP were reported to be effective for selective labelling K V 1 channels [ 61 ].…”

Section: Optical Probes Targeting Potassium Channelsmentioning

confidence: 99%

Neurotoxin-Derived Optical Probes for Biological and Medical Imaging

Ergen,

Shorter,

Ntziachristos

et al. 2023

Mol Imaging Biol

View full text Add to dashboard Cite

The superb specificity and potency of biological toxins targeting various ion channels and receptors are of major interest for the delivery of therapeutics to distinct cell types and subcellular compartments. Fused with reporter proteins or labelled with fluorophores and nanocomposites, animal toxins and their detoxified variants also offer expanding opportunities for visualisation of a range of molecular processes and functions in preclinical models, as well as clinical studies. This article presents state-of-the-art optical probes derived from neurotoxins targeting ion channels, with discussions of their applications in basic and translational biomedical research. It describes the design and production of probes and reviews their applications with advantages and limitations, with prospects for future improvements. Given the advances in imaging tools and expanding research areas benefiting from the use of optical probes, described here resources should assist the discovery process and facilitate high-precision interrogation and therapeutic interventions.

show abstract

Section: Optical Probes Targeting Potassium Channelsmentioning

confidence: 99%

Neurotoxin-Derived Optical Probes for Biological and Medical Imaging

Ergen,

Shorter,

Ntziachristos

et al. 2023

Mol Imaging Biol

View full text Add to dashboard Cite

show abstract

“…1, A, C). Among them are Cy3-, Cy5-or Alexa-labeled mutant HgTX1-A19C [2] and N-terminally labeled Atto488-HgTx1 [3]. However, the procedures of chemical labeling of peptides with fluorescent dyes have a number of limitations that often result in the low yield, as well as high cost of the labeled product.…”

Section: Moskva Russiamentioning

confidence: 99%

RFP-tagged Hongotoxin 1 and Its Interactions with KscA-Kv1.1 Hybrid Channels

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…1, B, F). To investigate the state of mKate2-Kv1.3 in plasma membrane we have applied a high affinity fluorescent ligand A-HgTx1 (HgTx1 labeled with Atto488), which was characterized by us previously [3]. A-HgTx1 is found to bind to plasma membrane of those cells, which demonstrate membrane expression of mKate2-Kv1.3 (Fig.…”

mentioning

confidence: 99%