Abstract:A rapid and convenient strategy to monitor the productivity of
biomanufacturing is essential for the research in optimizing relevant
bioprocesses. In this work, we have developed a fluorescein-derived
probe (FL-DT) that reacts rapidly with thiol groups via 1, 4-Micheal
addition reaction of the sulfhydryl to unsaturated ketone and releases
fluorescence. FL-DT specifically forms fluorescent adduct with two
adjacent thiols in a protein of interest (POI), making the probe a
reliable tool for protein quantification… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.