Fluorescent labeling of both GABAergic and glycinergic neurons in vesicular GABA transporter (VGAT)–Venus transgenic mouse

Wang, Y.; Kakizaki, Toshikazu; Sakagami, Hiroyuki; Saito, Keiichiro; Ebihara, Satoe; Kato, Megumi; Hirabayashi, Masumi; Saito, Yasuhiko; Furuya, Nobuhiko; Yanagawa, Yuchio

doi:10.1016/j.neuroscience.2009.09.010

Cited by 123 publications

(154 citation statements)

References 47 publications

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“…The two transgenic lines used to harvest MGE cells were VGAT-Venus mice (Line no. 39; Wang et al, 2009) or VGAT-ChR2(H134R)-EYFP from Jackson Laboratories (Zhao et al, 2011). Two C57Bl/6N mice studied by Video-EEG received transplants of E13.5 donor MGE cells from CD-1 pregnant dams (Charles River Laboratories).…”

Section: Methodsmentioning

confidence: 99%

“…Implanting GABA-releasing cell lines was found to elevate seizure thresholds through nonsynaptic mechanisms (Gernert et al, 2002;Thompson, 2009). Additional studies transplanting GABAergic progenitors from the embryonic forebrain medial ganglionic eminence (MGE) showed their capacity to migrate and differentiate into GABAergic interneurons (Wichterle et al, 1999;Alvarez-Dolado et al, 2006;Calcagnotto et al, 2010a,b). When transplanted into the adult rodent hippocampus, MGE cells reduced limbic seizures (Waldau et al, 2010;Hunt et al, 2013), increased inhibitory postsynaptic currents, and raised seizure thresholds (Calcagnotto et al, 2010a;Zipancic et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Long-Term Seizure Suppression and Optogenetic Analyses of Synaptic Connectivity in Epileptic Mice with Hippocampal Grafts of GABAergic Interneurons

et al. 2014

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Studies in rodent epilepsy models suggest that GABAergic interneuron progenitor grafts can reduce hyperexcitability and seizures in temporal lobe epilepsy (TLE). Although integration of the transplanted cells has been proposed as the underlying mechanism for these disease-modifying effects, prior studies have not explicitly examined cell types and synaptic mechanisms for long-term seizure suppression. To address this gap, we transplanted medial ganglionic eminence (MGE) cells from embryonic day 13.5 VGAT-Venus or VGATChR2-EYFP transgenic embryos into the dentate gyrus (DG) of adult mice 2 weeks after induction of TLE with pilocarpine. Beginning 3-4 weeks after status epilepticus, we conducted continuous video-electroencephalographic recording until 90 -100 d. TLE mice with bilateral MGE cell grafts in the DG had significantly fewer and milder electrographic seizures, compared with TLE controls. Immunohistochemical studies showed that the transplants contained multiple neuropeptide or calcium-binding protein-expressing interneuron types and these cells established dense terminal arborizations onto the somas, apical dendrites, and axon initial segments of dentate granule cells (GCs). A majority of the synaptic terminals formed by the transplanted cells were apposed to large postsynaptic clusters of gephyrin, indicative of mature inhibitory synaptic complexes. Functionality of these new inhibitory synapses was demonstrated by optogenetically activating VGAT-ChR2-EYFP-expressing transplanted neurons, which generated robust hyperpolarizations in GCs. These findings suggest that fetal GABAergic interneuron grafts may suppress pharmacoresistant seizures by enhancing synaptic inhibition in DG neural circuits.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Long-Term Seizure Suppression and Optogenetic Analyses of Synaptic Connectivity in Epileptic Mice with Hippocampal Grafts of GABAergic Interneurons

et al. 2014

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“…For two-photon imaging, adult (P56 -P112) male VGAT-Venus line #39 (Wang et al, 2009), GAD67-GFP (Tamamaki et al, 2003), and TH-GFP (Sawamoto et al, 2001;Matsushita et al, 2002) mice were used. In addition to these transgenic mice, adult (P56 -P62) male wild-type (C57BL/6J, purchased from SLC) and TH-Cre;Rosa26R-CFP (Srinivas et al, 2001;Gong et al, 2007) mice were used for immunohistological studies.…”

Section: Methodsmentioning

confidence: 99%

“…To observe the turnover dynamics of the entire PGC population under 2PLSM, we used adult VGAT-Venus mice, in which almost all the PGCs are labeled with Venus under the vesicular GABA transporter gene promoter (Wang et al, 2009) (Fig. 1 A-C; Table 1).…”

Section: Olfactory Input Promotes the Reiterated Use Of The Same Posimentioning

confidence: 99%

Sensory Input Regulates Spatial and Subtype-Specific Patterns of Neuronal Turnover in the Adult Olfactory Bulb

Sawada¹,

Kaneko²,

Inada³

et al. 2011

J. Neurosci.

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Throughout life, new neurons are added and old ones eliminated in the adult mouse olfactory bulb. Previous studies suggested that olfactory experience controls the process by which new neurons are integrated into mature circuits. Here we report novel olfactoryexperience-dependent mechanisms of neuronal turnover. Using two-photon laser-scanning microscopy and sensory manipulations in adult live mice, we found that the neuronal turnover was dynamically controlled by olfactory input in a neuronal subtype-specific manner. Olfactory input enhanced this turnover, which was characterized by the reiterated use of the same positions in the glomeruli by new neurons. Our results suggest that olfactory-experience-dependent modification of neuronal turnover confers structural plasticity and stability on the olfactory bulb.

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“…The mice older than P14 were male, although those younger than P12 were not identified as male or female. VGATVenus transgenic mice were generated as reported previously (Wang et al, 2009) and confirmed to express the normal level of the GABA synthesizing enzyme, glutamate decarboxylase 67 (Kameyama et al, 2010). In part of the experiments, we used CB1-KO mice.…”

Section: Methodsmentioning

confidence: 99%

Laminar-Specific Maturation of GABAergic Transmission and Susceptibility to Visual Deprivation Are Related to Endocannabinoid Sensitivity in Mouse Visual Cortex

Jiang¹,

Sohya²,

Sarihi³

et al. 2010

J. Neurosci.

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The developmental period when neuronal responses are modified by visual experience is reported to start and end earlier in layer 4 than in layer 2/3ofthevisualcortex,andthematurationofGABAergicinhibitorycircuitsissuggestedtodeterminethetimingofthisperiod.Here,weexamine whether the laminar difference in such timing corresponds to a difference in the time course of the functional maturation of GABAergic synaptic transmission to star pyramidal and pyramidal cells in layers 4 and 2/3, respectively, of the mouse visual cortex and whether the development of the strength of GABAergic transmission is affected by visual deprivation in a laminar-specific manner. Our analysis of developmental changes in inhibitory postsynaptic currents of star pyramidal and pyramidal cells evoked by electrical stimulation of afferents or action potentials of fast-spiking GABAergic neurons revealed that there was a sequential maturation of GABAergic function from layers 4 to 2/3. The maturation of inhibition in layer 4 occurred at postnatal week 3, which preceded by 1 week that of layer 2/3. Visual deprivation by dark rearing arrested the functional development of GABAergic transmission in layer 2/3, whereas dark rearing was not so effective in layer 4. GABAergic synapses in layer 2/3 were sensitive to an agonist for cannabinoid type 1 receptors and not normally matured in receptor knock-out mice, whereas those in layer 4werenotso.Theseresultssuggestlaminar-specificmaturationofinhibitionandsusceptibilitytovisualdeprivation,whichmayberelatedtothe laminar difference in sensitivity to endocannabinoids.

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Fluorescent labeling of both GABAergic and glycinergic neurons in vesicular GABA transporter (VGAT)–Venus transgenic mouse

Cited by 123 publications

References 47 publications

Long-Term Seizure Suppression and Optogenetic Analyses of Synaptic Connectivity in Epileptic Mice with Hippocampal Grafts of GABAergic Interneurons

Long-Term Seizure Suppression and Optogenetic Analyses of Synaptic Connectivity in Epileptic Mice with Hippocampal Grafts of GABAergic Interneurons

Sensory Input Regulates Spatial and Subtype-Specific Patterns of Neuronal Turnover in the Adult Olfactory Bulb

Laminar-Specific Maturation of GABAergic Transmission and Susceptibility to Visual Deprivation Are Related to Endocannabinoid Sensitivity in Mouse Visual Cortex

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