Fluorescent label-free quantitative detection of nano-sized bioparticles using a pillar array

Zeming, Kerwin Kwek; Salafi, Thoriq; Shikha, Swati; Zhang, Yong

doi:10.1038/s41467-018-03596-z

Cited by 48 publications

(33 citation statements)

References 55 publications

(48 reference statements)

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“…With the development of DLD technology, there are multiple modes of DLD applications that are mostly demonstrated for medical and biological research applications, including the particle separation, concentration, buffer exchange, and [23] label-free detection. Initially, DLD uses the particle size parameter for the basis of the separation.…”

Section: Dld Applicationsmentioning

confidence: 99%

A Review on Deterministic Lateral Displacement for Particle Separation and Detection

2019

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HIGHLIGHTS• A well-organized and thorough discussion on the fundamental principles and recent progress in deterministic lateral displacement (DLD) is provided.• The most updated designs and applications of DLD techniques for particle separation and detection are reviewed.• The current limitations of DLD and its potential solutions for clinical and commercial applications are discussed.ABSTRACT The separation and detection of particles in suspension are essential for a wide spectrum of applications including medical diagnostics. In this field, microfluidic deterministic lateral displacement (DLD) holds a promise due to the ability of continuous separation of particles by size, shape, deformability, and electrical properties with high resolution. DLD is a passive microfluidic separation technique that has been widely implemented for various bioparticle separations from blood cells to exosomes. DLD techniques have been previously reviewed in 2014. Since then, the field has matured as several physics of DLD have been updated, new phenomena have been discovered, and various designs have been presented to achieve a higher separation performance and throughput. Furthermore, some recent progress has shown new clinical applications and ability to use the DLD arrays as a platform for biomolecules detection. This review provides a thorough discussion on the recent progress in DLD with the topics based on the fundamental studies on DLD models and applications for particle separation and detection. Furthermore, current challenges and potential solutions of DLD are also discussed. We believe that a comprehensive understanding on DLD techniques could significantly contribute toward the advancements in the field for various applications. In particular, the rapid, low-cost, and high-throughput particle separation and detection with DLD have a tremendous impact for point-of-care diagnostics.

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Section: Dld Applicationsmentioning

confidence: 99%

A Review on Deterministic Lateral Displacement for Particle Separation and Detection

2019

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“…Amongst the fluid-mediated surface interactions between solids, electrostatic forces are expected to be of primary importance. In the specific context of DLD devices, recent articles by Zeming et al [33,34] showed that particle-obstacle electrostatic interactions can significantly impact the dynamics of suspended particles in DLD arrays. These interactions originate from the existence of an Electric Double Layer (EDL) at the solid-fluid interface.…”

Section: Electrostatic Forcesmentioning

confidence: 99%

Combining Electrostatic, Hindrance and Diffusive Effects for Predicting Particle Transport and Separation Efficiency in Deterministic Lateral Displacement Microfluidic Devices

et al. 2020

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Microfluidic separators based on Deterministic Lateral Displacement (DLD) constitute a promising technique for the label-free detection and separation of mesoscopic objects of biological interest, ranging from cells to exosomes. Owing to the simultaneous presence of different forces contributing to particle motion, a feasible theoretical approach for interpreting and anticipating the performance of DLD devices is yet to be developed. By combining the results of a recent study on electrostatic effects in DLD devices with an advection–diffusion model previously developed by our group, we here propose a fully predictive approach (i.e., ideally devoid of adjustable parameters) that includes the main physically relevant effects governing particle transport on the one hand, and that is amenable to numerical treatment at affordable computational expenses on the other. The approach proposed, based on ensemble statistics of stochastic particle trajectories, is validated by comparing/contrasting model predictions to available experimental data encompassing different particle dimensions. The comparison suggests that at low/moderate values of the flowrate the approach can yield an accurate prediction of the separation performance, thus making it a promising tool for designing device geometries and operating conditions in nanoscale applications of the DLD technique.

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“…The microfluidics DLD based on the asymmetrical gap with a lateral gap of 9 µm and downstream gap of 3 µm from our previous work was used to demonstrate the microbeads separation and detection on the smartphone platform (Figure 9, Supporting Information). [21,47,48] The device was made from PDMS material and fabricated through soft lithography process from SU-8 on silicon master mold. [21,47,48] The device was made from PDMS material and fabricated through soft lithography process from SU-8 on silicon master mold.…”

Section: Wwwadvmattechnoldementioning

confidence: 99%

Portable Smartphone‐Based Platform for Real‐Time Particle Detection in Microfluidics

Salafi

Zeming

Lim

et al. 2018

Adv Materials Technologies

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Real‐time particle detection is an integral part of microfluidics for diagnostics. The detection method is typically performed with the use of bulky and expensive instruments including a bench‐top microscope, high‐speed camera, and fluidic pump. This limits the practical applications of microfluidics for real‐time point‐of‐care detection. Here, a portable and low‐cost smartphone microscopy platform is developed for real‐time particle detection in microfluidics based on a polydimethylsiloxane (PDMS) lens, modular 3D printed accessory, plug‐and‐play paper pump, and a novel particle counting algorithm. The PDMS lens is developed using a new method based on the PDMS precuring and hydrophobic modification on a pillar template to allow for fabrication of various lens shapes with high reproducibility and broad range magnification ratio from 30× to 100×. The modular 3D printed smartphone accessory allows for easy imaging setup to cater for different size of microchannel and the paper pump design provides a convenient single‐step process to drive the fluid. The platform is implemented to count the moving particles in the outlet of microfluidics deterministic lateral displacement with 94% counting accuracy. This platform provides huge potential applications for real‐time point‐of‐care detection that can be integrated with various microfluidic techniques.

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Fluorescent label-free quantitative detection of nano-sized bioparticles using a pillar array

Cited by 48 publications

References 55 publications

A Review on Deterministic Lateral Displacement for Particle Separation and Detection

A Review on Deterministic Lateral Displacement for Particle Separation and Detection

Combining Electrostatic, Hindrance and Diffusive Effects for Predicting Particle Transport and Separation Efficiency in Deterministic Lateral Displacement Microfluidic Devices

Portable Smartphone‐Based Platform for Real‐Time Particle Detection in Microfluidics

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