Fluorescent intracellular imaging of reactive oxygen species and pH levels moderated by a hydrogenase mimic in living cells

Hu, Xinyuan; Li, Jiajing; Yang, Zi-Wei; Zhang, Jun; Wang, Huai-Song

doi:10.1016/j.jpha.2022.05.007

Cited by 5 publications

(1 citation statement)

References 38 publications

(40 reference statements)

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“…4 With the continuous development of optical imaging technology for decades, it has been widely used in the preclinical diagnosis and treatment of cancer in biomedical research. [5][6][7][8] Nanomaterials for optical imaging, such as nano clusters, 9 silicon dioxide, carbon dots and magnetic materials, 10,11 have always been the focus of cancer diagnosis research because of their uniqueness. 12 The intrinsic optical properties of nanoparticles make them quickly become the best choice for various cell imaging modes.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Application of a Near-Infrared Light-Emitting Fluorescent Probe for Specific Imaging of Cancer Cells with High Sensitivity and Selectivity

Li,

Lin,

Chen

et al. 2024

DDDT

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Background The preclinical diagnosis of tumors is of great significance to cancer treatment. Near-infrared fluorescence imaging technology is promising for the in-situ detection of tumors with high sensitivity. Methods Here, a fluorescent probe was synthesized on the basis of Au nanoclusters with near-infrared light emission and applied to fluorescent cancer cell labeling. Near-infrared methionine-N-Hydroxy succinimide Au nanoclusters (Met-NHs-AuNCs) were prepared successfully by one-pot synthesis using Au nanoclusters, methionine, and N-Hydroxy succinimide as frameworks, reductants, and stabilizers, respectively. The specific fluorescence imaging of tumor cells or tissues by fluorescent probe was studied on the basis of SYBYL Surflex-DOCK simulation model of LAT1 active site of overexpressed receptor on cancer cell surface. The results showed that Met-NHs-AuNCs interacted with the surface of LAT1, and C_Score scored the conformation of the probe and LAT1 as five. Results Characterization and in vitro experiments were conducted to explore the Met-NHs-AuNCs targeted uptake of cancer cells. The prepared near-infrared fluorescent probe (Met-NHs-AuNCs) can specifically recognize the overexpression of L-type amino acid transporter 1 (LAT1) in cancer cells so that it can show red fluorescence in cancer cells. Meanwhile, normal cells (H9c2) have no fluorescence. Conclusion The fluorescent probe demonstrates the power of targeting and imaging cancer cells.

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