Fluorescent in situ hybridization as a screening test for HER2 amplification in G2 and G3 breast cancers of lobular and ductal histotype and metastases

Castellano, Isabella; Sapino, Anna; Arisio, Riccardo; Viale, Giuseppe; Bussolati, Gianni; Bandelloni, Roberto; Barresi, G; Bersiga, Alessandra; Bordi, Cesare; Botti, Gerardo; Cosimi, F; d'Amore, Emanuele S G; Doglioni, Claudio; Marchetti, Antonio; Nappi, Oscar; Romeo, Francesco; Roncalli, Massimo; Russo, Rosa; Santinelli, Alfredo; Spagnoli, Luigi Giusto; Tanda, Francesco; Tricomi, Paolo; Trentini, Gianpaolo; Zanconati, Fabrizio; Iurlaro, Monica

doi:10.3892/or.19.5.1271

Cited by 2 publications

(2 citation statements)

References 29 publications

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“…14,15 Tissue macro arrays were prepared with approximately four spots from each significant areas of all cases. Each tissue macro array was FISH tested with selfmade red-labelled p63-specific locus probe and green chromosome 3 alpha satellite control probe (Cytocell Tehcnologies, UK).…”

Section: Fish Analysismentioning

confidence: 99%

Expression of p63 is the sole independent marker of aggressiveness in localised (stage I–II) Merkel cell carcinomas

et al. 2011

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Merkel cell carcinoma of the skin is a malignant neuroendocrine tumour, whose prognostic criteria are a matter of dispute. Specifically, no predictor is presently available in stage I-II tumours. We collected clinical and follow-up data from 70 Merkel cell carcinomas of the skin. The same cases were studied for p63 expression by immunohistochemistry, by reverse-transcription PCR (RT-PCR) and TP63 gene status by FISH and for presence of Merkel cell polyomavirus by PCR. Stage emerged as a significant prognostic parameter (P ¼ 0.008). p63 expression, detected in 61% (43/70) of cases by immunohistochemistry, was associated with both decreased overall survival (Po0.0001) and disease-free survival (Po0.0001). Variable expression patterns of the different p63 isoforms were found only in cases immunoreactive for p63. In these latter lesions, at least one of the N-terminal p63 isoforms was detected and TAp63a was the most frequently expressed isoform. TP63 gene amplification was observed by FISH in only one case. Presence of Merkel cell polyomavirus DNA sequences was detected in 86% (60/70) of Merkel cell carcinomas and did not emerge as a significant prognostic parameter. Merkel cell carcinoma cases at low stage (stage I-II) represented over half (40/70 cases, 57%) of cases, and the clinical course was uneventful in 25 of 40 cases while 15 cases died of tumour (10/40 cases) within 34 months or were alive with disease (5/40 cases) within 20 months. Interestingly, a very strict correlation was found between evolution and p63 expression (Po0.0001). The present data indicate that p63 expression is associated with a worse prognosis in patients with Merkel cell carcinoma, and in localised tumours it represents the single independent predictor of clinical evolution.

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Section: Fish Analysismentioning

confidence: 99%

Expression of p63 is the sole independent marker of aggressiveness in localised (stage I–II) Merkel cell carcinomas

et al. 2011

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“…31 Briefly, sections were incubated overnight at 561C, dewaxed in xylene, dehydrated in 100% ethanol and airdried. Slides were then treated with proteases for 45-60 min, denatured at 971C, and hybridized overnight at 371C.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Micropapillary ductal carcinoma in situ of the breast: an inter-institutional study

et al. 2010

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The clinical significance of micropapillary growth pattern in ductal carcinoma in situ is controversial and the impact of nuclear grading in terms of recurrence of this lesion is yet to be clarified. Our aim was to evaluate, on a series of micropapillary in situ carcinomas, the histological features correlated with recurrence and whether the micropapillary subtype had a different behavior from other non-micropapillary ductal carcinoma in situ. We collected 55 cases of micropapillary in situ carcinomas from four institutions. All cases were reviewed for nuclear grade, extent, necrosis, microinvasion and tested for estrogen and progesterone receptors, Ki67, HER2, EGFR and p53 expression. Clinical data, type of surgery and follow up were obtained for all patients. Our results showed that the nuclear grade is crucial in determining the biology of micropapillary carcinoma in situ, so that the high nuclear grade micropapillary ductal carcinoma in situ more frequently overexpressed HER2, showed higher proliferation index, displayed necrosis and microinvasion and was more extensive than low/intermediate nuclear grade. Logistic regression analysis confirmed the high nuclear grade (Odds ratio: 6.86; CI: 1.40-33.57) as the only parameter associated with elevated risk of local recurrence after breast-conserving surgery. However, the recurrence rate of 19 micropapillary carcinoma in situ, which were part of a cohort of 338 consecutive ductal carcinoma in situ, was significantly higher (log-rank test, P-value ¼ 0.019) than that of non-micropapillary, independently of the nuclear grade. In conclusion, although nuclear grade may significantly influence the biological behavior of micropapillary ductal carcinoma in situ, micropapillary growth pattern per se represents a risk factor for local recurrence after breast-conserving surgery.

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Fluorescent in situ hybridization as a screening test for HER2 amplification in G2 and G3 breast cancers of lobular and ductal histotype and metastases

Cited by 2 publications

References 29 publications

Expression of p63 is the sole independent marker of aggressiveness in localised (stage I–II) Merkel cell carcinomas

Expression of p63 is the sole independent marker of aggressiveness in localised (stage I–II) Merkel cell carcinomas

Micropapillary ductal carcinoma in situ of the breast: an inter-institutional study

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