Fluorescent In Situ Hybridization and Micro‐autoradiography Applied to Ecophysiology in Soil

Rogers, Shane; Moorman, Thomas B.; Ong, Say Kee

doi:10.2136/sssaj2006.0105

Cited by 64 publications

(12 citation statements)

References 123 publications

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“…In bacteria, this sensitive technique has been used to study metabolic pathways by using radiolabeled media; however, dynamic SIMS imaging is quickly becoming an attractive alternative to this technique. When applied to soil ecophysiology, this technique combined with FISH was able to correlate specific nutrient uptake with the identity of the microbe 86 . Similar experiments have been done using FISH and SIMS IMS 34,35,72–75 .…”

Section: Box 1: Alternate Imaging Techniquesmentioning

confidence: 99%

Imaging mass spectrometry in microbiology

2011

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Mass spectrometry tools which allow for the 2-D visualization of the distribution of trace metals, metabolites, surface lipids, peptides and proteins directly from biological samples without the need for chemical tagging or antibodies are becoming increasingly useful for microbiology applications. These tools, comprised of different imaging mass spectrometry techniques, are ushering in an exciting new era of discovery by allowing for the generation of chemical hypotheses based on of the spatial mapping of atoms and molecules that can correlate to or transcend observed phenotypes. In this review, we explore the wide range of imaging mass spectrometry techniques available to microbiologists and describe their unique applications to microbiology with respect to the types of microbiology samples to be investigated.

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Section: Box 1: Alternate Imaging Techniquesmentioning

confidence: 99%

Imaging mass spectrometry in microbiology

2011

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“…Recently, there has been an explosion in the identification of microorganisms in the natural world using cultureindependent techniques. The structure and function of these microbial communities using new molecular technologies can be determined (Mills et al, 2007;Thies, 2007;Nakatsu, 2007;Rogers et al, 2007;Suzuki et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Influence of irrigated agriculture on soil microbial diversity

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Mills

Mathee

et al. 2008

Applied Soil Ecology

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“…However, it can be coupled with other techniques such as microautoradiography to describe functional properties of microorganisms in their natural environment (Wagner et al 2006). Many improvements have been made in FISH analysis to solve the problems associated with it: Bright fluorochromes, hybridization with the probes carrying multiple fluorochromes, treatment with chloramphenicol to increase the RNA content of the cells (Rogers et al 2007), or addition of nutrients to stimulate bacterial activity (Hahn et al 1992). The low signal intensity, target inaccessibility, and background fluorescence are the common problems associated with FISH analysis.…”

Section: Intermediate Resolution Methodsmentioning

confidence: 99%