Fluorescent in situ hybridization and characterization of the SalI family of satellite repeats in the Haliotis L. species (abalone) of the Northeast Pacific

Abstract

The SalI satellite repeat previously identified in Haliotis L. (abalone) was thought to be present in H. rufescens and absent in H. fulgens. However, we show here that SalI is also found in H. fulgens and is not useful for screening hybrid individuals bred in aquaculture or occurring naturally in the wild. SalI is a family of predominantly subtelomeric tandemly repeated sequences, and sequenced clones revealed clustering to species and little intraspecific variation. Analysis of SalI sequence divergence between Haliotis species of the Northeast Pacific revealed that evolutionary distances correlate well with bathymetric and latitudinal species distributions. Analysis of the structure of the tandem repeats revealed two regions of high sequence conservation that may contain conserved transcription factor binding sites, a surprise for an apparently "non-coding" tandem repeat. We speculate that these regions might be involved in heterochromatin silencing, perhaps mediated via transcriptional activity and RNA interference. The repeats show substantial differences in their chromosomal distributions, even between individuals of the same species, indicating a dynamic organization of repeats, perhaps mediated via sequence homogenization.

Dampak Artificial Intelligence Terhadap Perekonomian

“…"Masyarakat dengan biaya marjinal nol" ini telah lama digembargemborkan sebagai model bisnis baru (Rifkin, 2014), tetapi semakin menunjukkan keterbatasannya baik dalam hal masalah privasi orang maupun dalam hal dampak ekonomi dan sosialnya, seperti yang telah dibahas sebelumnya. Salah satu solusi untuk mengatasi masalah ini adalah konsumen terus membagikan data mereka secara bebas tetapi membatasi penggunaannya untuk tujuan tertentu yang hanya memberikan peluang keuntungan yang terbatas (Arrieta- Ibarra. et al;.…”

Telomeres and genomic evolution

“…The occurrence of variable repeat sequence families is a generalized property of sub-telomeric regions across a broad range of species [94][95][96][97][98]. It appears that these regions of the genome have a propensity to accumulate repetitive DNA, with ectopic recombination being invoked as the key driver of this variation.…”

Intraspecies and interspecies hybrids in Haliotis: natural and experimental evidence and its impact on abalone aquaculture

“…However, it was absent from the black and green species and it was suggested that this DNA satellite sequence could be used to identify hybrids. However, Hernández- Ibarra et al (2008) discovered that SalI is also found in green abalone (H. fulgens) and is not useful for screening hybrid individuals between H. rufescens and H. fulgens. Furthermore, they produced the hybrid H. rufescens $ · H. fulgens # and identified both parental chromosomes in the hybrid offspring using genomic in situ hybridization (GISH), indicating that this technique permits accurate determination of the putative hybrid nature of early breeding material ).…”

“…However, it was absent from the black and green species and it was suggested that this DNA satellite sequence could be used to identify hybrids. However, Hernández‐Ibarra et al. (2008) discovered that SalI is also found in green abalone ( H. fulgens ) and is not useful for screening hybrid individuals between H. rufescens and H. fulgens .…”